Phytochemistry of Ziziphus Mauritiana; its Antioxidant and Antimicrobial Potential

Mohsin Javed, Rabiya Bibi, Khalida Nazir, Shabbir Hussain


Background: Z. mauritiana is a very common and easily available plant all over the world, including Pakistan. It finds a lot of importance due to its nutritional and medicinal value.

Methods: Current studies were performed to investigate the phytochemical nature and antioxidant and antimicrobial potential of Z. mauritiana (Lahore, Pakistan). Total phenolic contents in leaves and pulp extracts were identified using a modified Folin-Ciocalteu’s method while total sugars were determined by Lane and Eynon method. Antioxidant activity was determined by DPPH radical-scavenging activity assay using a UV-Visible spectrophotometer at 517 nm. The antimicrobial potential was tested by agar well diffusion method using imipenem as standard drug.

Results: The methanolic extracts of leaves and pulp contained tannins, saponins, phlobatannins, alkaloids and flavonoids while steroids were absent in both the samples. The pulp and leave extracts have shown the presence of 57.3% and 37.89% sugar contents, respectively. The concertation of total phenolic contents (TPC) was found higher in pulp extracts as compared to that observed in leaves. The methanolic pulp extract has shown higher antioxidant potential as compared to the leaves extract. However, methanolic leaves extract exhibited higher antibacterial potential as compared to the methanolic pulp extract.

Conclusion: The methanolic extracts of Z. Mauritiana pulp and leaves contain many biologically active ingredients and are biologically active against most of the tested bacterial strains. Its pulp and leaves are richer in numerous antioxidants and secondary metabolites. The pulp extract exhibited higher antioxidant potential as compared to the leaves extract.

Keywords: Z. Mauritiana; Phytochemicals; Total Phenolic contents; Antibacterial 

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Naseer S, Hussain S, Naeem N, Pervaiz M, Rahman M. The phytochemistry and medicinal value of Psidium guajava (guava). Clinical Phytoscience, (2018); 4(1): 1-8.

Rehman A, Hussain S, Javed M, Ali Z, Rehman H, Shahzady TG, Zahra A. Chemical composition and remedial perspectives of Hippophae rhamnoides linn. Postepy Biologii Komorki, (2018); 45(3): 199-209.

Farhat N, Hussain S, Syed SK, Amjad M, Javed M, et al. Dietary phenolic compounds in plants; their antioxidant and pharmacological potential. Postepy Biologii Komorki, (2020); 47(3): 307-20.

Najafi S, Sanadgol N, Nejad BS, Beiragi MA, Sanadgol E. Phytochemical screening and antibacterial activity of Citrullus colocynthis (Linn.) Schrad against Staphylococcus aureus. Journal of Medicinal Plants Research, (2010); 4(22): 2321-2325.

Amjad M, Hussain S, Javaid K, Khan A, Ali B, Noreen M, et al. Plant representation, phytochemistry and medicinal assets of Asparagus racemosus. The Pharmaceutical and Chemical Journal, (2020); 7(5): 12-23.

Naseer S, Hussain S, Zahid Z. Nutritional and Antioxidant Potential of Common Vegetables in Pakistan. RADS Journal of Biological Research & Applied Sciences, (2019); 10(1): 36-40.

Petrovska BB. Historical review of medicinal plants’ usage. Pharmacognosy reviews, (2012); 6(11): 1.

Yuan H, Ma Q, Ye L, Piao G. The traditional medicine and modern medicine from natural products. Molecules, 2016; 21(5): 559.

Vaidya AD, Devasagayam TP. Current status of herbal drugs in India: an overview. Journal of clinical biochemistry and nutrition, (2007); 41(1): 1-11.

Ahmad M, Zafar M, Sultana S. Salvadora persica, Tamarix aphylla and Zizyphus mauritiana-three woody plant species mentioned in Holy Quran and Ahadith and their ethnobotanical uses in north western part (DI Khan) of Pakistan. Pakistan Journal of Nutrition, (2009); 8(5): 542-547.

Butt SZ, Hussain S, Munawar KS, Tajammal A, Muazzam MA. Phytochemistry of Ziziphus Mauritiana; its Nutritional and Pharmaceutical Potential. Scientific Inquiry and Review, (2021); 5(2): 1-15.

Sareen A, Gupta RC, Bansal G, Singh V. Comparison of key mineral elements in wild edible fruits of Ziziphus Mauritiana and Z. nummularia using atomic absorption spectrophotometer (AAS) and flame photometer. International Journal of Fruit Science, (2020); 20(sup2): S987-S994.

Goyal M, Nagori BP, Sasmal D. Review on ethnomedicinal uses, pharmacological activity and phytochemical constituents of Ziziphus Mauritiana (Z. jujuba Lam., non Mill). Spatula DD, (2012); 2(2): 107-116.

Adzu B, Amos S, Wambebe C, Gamaniel K. Antinociceptive activity of Zizyphus spina-christi root bark extract. Fitoterapia, (2001); 72(4): 344-350.

Parmar P, Bhatt S, Dhyani S, Jain A. Phytochemical studies of the secondary metabolites of Ziziphus Mauritiana Lam. Leaves. International journal of current pharmaceutical research, (2012); 4(3): 153-155.

Nyanga LK, Nout MJ, Gadaga TH, Theelen B, Boekhout T, Zwietering MH. Yeasts and lactic acid bacteria microbiota from masau (Ziziphus Mauritiana) fruits and their fermented fruit pulp in Zimbabwe. International journal of food microbiology, (2007); 120(1): 159-166.

Huang YL, Yen GC, Sheu F, Chau CF. Effects of water-soluble carbohydrate concentrate from Chinese jujube on different intestinal and fecal indices. Journal of agricultural and food chemistry, (2008); 56(5): 1734-1739.

Sherani J, Jilani MS, Ahmad T, Kamaran S, Manan A, Jilani TA, Sajid M. Local Rootstock of Ber (Ziziphus Mauritiana L) Grafted with Advanced Scion Cultivars Improved Fruit Yield and Quality under Arid Conditions. Sarhad Journal of Agriculture, (2021); 37(2): 492: 499.

Pareek O. Fruits for the future 2: Ber. International Centre for Underutilized Crops, University of Southampton, Southampton, UK, 2001; 290.

Marwat SK, Khan MA, Khan MA, Ahmad M, Zafar M, et al. Fruit plant species mentioned in the Holy Qura’n and Ahadith and their ethno medicinal importance. American-Eurasian Journal of Agricultural and Environmental Science, (2009); 5(2): 284-295.

Al-Hilali M, Khan MM. The Noble Ouran: English Translation of the meaning and commentary. King Fahd Complex for the printing of Holy Ouran Madinah, KSA, 1985; 13.

Farooqi I. Ahadith mein mazkoor nabatat, adwiya aur ghizain. Ilm-o-Irfan Pulishers, 1998; 38.

Chughtai T. Nabatat-e-Qurani Aur Jadid Science. Dar-ul-Isha’t Urdu Bazar Karachi, 2000; 101: 449.

Mukhtar HM, Ansari S, Ali M, Naved T. New compounds from Zizyphus vulgaris. Pharmaceutical Biology, 2004; 42(7): 508-511.

Meena S, Meena H, Meena R. Diversified uses of ber (Ziziphus spp.). Popular Kheti, (2014); 2(1): 154-159.

Parmar P, Bhatt S, Dhyani S, Jain A. Phytochemical studies of the secondary metabolites of Ziziphus Mauritiana Lam. Leaves. IInternational Journal of Current Pharmaceutical Research, (2012); 4(3): 153-155.

Morton J. Indian jujube. Fruits of warm climates, 1987: 272-275.

Dahiru D, Sini J, John-Africa L. Antidiarrhoeal activity of Ziziphus Mauritiana root extract in rodents. African journal of biotechnology, (2006); 5(10): 941-945.

Kushwaha P, Yadav SS, Singh V, Dwivedi L. GC-MS analysis of bio-active compounds in methanolic extract of Ziziphus Mauritiana fruit. International Journal of Pharmaceutical Sciences and Research, (2019); 10(6): 2911-2916.

Abalaka M, Daniyan S, Mann A. Evaluation of the antimicrobial activities of two Ziziphus species (Ziziphus Mauritiana L. and Ziziphus spinachristi L.) on some microbial pathogens. African Journal of Pharmacy and Pharmacology, (2010); 4(4): 135-139.

Amin I, Norazaidah Y, Hainida KE. Antioxidant activity and phenolic content of raw and blanched Amaranthus species. Food Chemistry, (2006); 94(1): 47-52.

Lane JH. Determination of reducing sugar by means of Fehling's solution with methylene blue as internal indicator. Journal of the Society of Chemical Industry, (1923); 17: 32-37.

Espín JC, Soler-Rivas C, Wichers HJ. Characterization of the total free radical scavenger capacity of vegetable oils and oil fractions using 2, 2-diphenyl-1-picrylhydrazyl radical. Journal of Agricultural and Food Chemistry, (2000); 48(3): 648-656.

Naseer S, Afzal M, Nisa A, Hussain S, Ahmad M, et al. Extraction of brown dye from Eucalyptus bark and its applications in food storage. Quality Assurance and Safety of Crops & Foods, (2019); 11(8): 769-780.

Javed M, Shoaib M, Iqbal Z, Khan MA, Hussain S, Amjad M. Phytochemical and Biological Studies on Curcuma longa L. in Pattoki (Kasur), Pakistan: Chemical and Biological studies of Curcuma longa. Proceedings of the Pakistan Academy of Sciences: B Life and Environmental Sciences, (2020); 57(2): 59-66.

Sanja S, Sheth N, Patel N, Patel D, Patel B. Characterization and evaluation of antioxidant activity of Portulaca oleracea. International Journal of Pharmacy and Pharmaceutical Sciences, (2009); 1(1): 5-10.

Kedare SB, Singh R. Genesis and development of DPPH method of antioxidant assay. Journal of food science and technology, (2011); 48(4): 412-422.

Sivasankari M, Sankaravadivoo A. Studies on antimicrobial activity of Ziziphus Mauritiana lam. International Journal of Ayurveda and Pharma Research, (2015); 3(7): 52-55.

Siddiqua A, Hussain S, Syed SK. Phytochemistry, nutritional and medicianl importance of almond. Postepy Biologii Komorki, (2021); 48(2): 167-180.

Rasheed RB, Hussain S, Syed SK. Phytochemistry, nutritional and medicianl value of kiwi fruit. Postepy Biologii Komorki, (2021); 48(2): 147-165.

Riaz M, Fatima H, ur Rehman MM, Qadir R, Hussain S, et al. Appraisal of antioxidant potential and biological studies of bogan bail (Bougainvillea glabra) leaf extracts using different solvents. Czech Journal of Food Sciences, (2021); 39(3): 176-180.


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