Medicinal Properties of Arabica coffee (Coffea arabica) Oil: An Overview

Khalid Mushabbab ALAsmari, Isam M Abu Zeid, Atef M Al-Attar


Coffee is one of the massive tropical crops in developing countries and historically understudied in subjects of crop nutrition and administration. Arabian coffee (Coffea arabica) plant belongs to the genus Coffea in the Rubiaceae family. It is known as the most widely recognized Coffea species created comprehensively summing up to over 75% of the all-out Coffea creation. Its compounds are a complex mixture of different chemicals that have many health benefits. The usage of various parts of a coffee plant, along with its oil is verified for the manufacturing of ancient medicines that helped in curing a number of ailments. These traditional uses were scientifically proven by many studies including psychoactive responses, neurological and metabolic disorders. Coffee oil consists mainly of triglycerol and fatty acids along with antioxidants. It also possesses some biologically active fatty acids that are anti-cancerous, anti-inflammatory, anti-bacterial, anti-diabetic and anti-atherosclerotic in nature. This paper provides the medicinal properties and scientific review of Arabica coffee Oil.

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Almalki DA, Alghamdi SA, Al-Attar AM. Comparative Study on the Influence of Some Medicinal Plants on Diabetes Induced by Streptozotocin in Male Rats. Biomed Research International, (2019); 3596287: 1-11.

Cos P, Vlietinck AJ, Berghe DV, Maes L. Anti-infective potential of natural products: How to develop a stronger in vitro proof-of-concept. Journal of Ethnopharmacology, (2006); 106 (3): 290-302.

McLain KA, Miller KA, Collins WR. Introducing organic chemistry students to natural product isolation using steam distillation and liquid phase extraction of thymol, camphor and citral, monoterpenes sharing a unified biosynthetic precursor. Journal of Chemical Education, (2015); 92 (7): 1226-1228.

Azwanida N N. A review on the extraction methods use in medicinal plants, principle, strength and limitation. Medicinal and Aromatic Plants, (2015), 4(3): 196-202.

Maurin O, Davis AP, Chester M, Mvungi EF, Jaufeerally-Fakim Y, et al. Towards a phylogeny for coffea (rubiaceae): identifying well-supported lineages based on nuclear and plastid DNA sequences. Annals of Botany, (2007); 100(7): 1565–83.

Kusolwa PM, Makwinja F, Nashon J, Marianna M, Kibola A. Morphological diversity of wild coffee (Coffea kihansiensis) a potential coffee species for genetic improvement. Tanzania Journal of Science, (2019); 45(4): 629-649.

Bresciani L, Calani L, Bruni R, Brighenti F, Rio DD. Phenolic composition, caffeine content and antioxidant capacity of coffee silverskin. Food Research International, (2014); 61: 196–201.

Murthy PS, Naidu MM. Sustainable management of coffee industry by-products and value addition-a review. Resources, Conservation and Recycling, (2012); 66: 45–58.

Jenkins RW, Stageman NE, Fortune CM. Chuck CJ. Effect of the type of bean, processing, and geographical location on the biodiesel produced from waste coffee grounds. Energy Fuels, (2014); 28(2): 1166–1174.

Dang CH, Nguyen TD. Physicochemical characterization of robusta spent coffee ground oil for biodiesel manufacturing. Waste and Biomass Valorization, (2019); 10: 2703–2712.

Pohlan HAJ, Janssens MJ. Growth and production of coffee, in Soils, Plant Growth and Crop Production, ed. by W.H. Verheye EOLSS Publications, Abu Dhabi, (2010); pp. 102–134.

Feyzioglu G F, Tornuk F. Development of chitosan nanoparticles loaded with summer savory (Satureja hortensis L.) essential oil for antimicrobial and antioxidant delivery applications. LWT – Food Science and Technology, (2016); 70: 104-110.

Castro A, Oda F, Almeida M, Davanço M, Chiari B, et al . Green coffee seed residue: A sustainable source of antioxidant compounds. Food Chemistry, (2018), 246: 48–57.

Pereda MDCV, Dieamant G C, Eberlin S, Nogueira C, Colombi D, Di Stasi LC, et al. Effect of green Coffea arabica L. seed oil on extracellular matrix components and water-channel expression in in vitro and ex vivo human skin models. Journal of Cosmetic Dermatology, (2009); 8(1): 56-62.

Bisht S, Sisodia SS. Coffea arabica: A wonder gift to medical science. Journal of Natural Pharmaceuticals, (2010); 1 (1):58-65.

Klinkesorn U, Sophanodora P, Chinachoti P, McClements DJ, Decker EA. Increasing the oxidative stability of liquid and dried tuna oil-in-water emulsions with electrostatic layer-by-layer deposition technology. Journal of Agricultural and Food Chemistry, (2005); 53 (11): 4561–4566.

Lorente-Cebrián S, Costa AG, Navas-Carretero S, Zabala M, Laiglesia LM, et al. An update on the role of omega-3 fatty acids on inflammatory and degenerative diseases. Journal of Physiology and Biochemistry, (2015); 71: 341–349.

Haarer A E. Modern coffee production. Leonard Hill, London.1962.

Wrigley G. Coffee. – Tropical agriculture series, Longmans Scientific and Technical, Harlow.1988

Pendergrast, M. Uncommon grounds: the history of coffee and how it transformed our world. – Basic Books, Perseus Books Groups, New York.1999.

Anthony F, Bertrand B, Etienne H, Lashermesm P. Coffea and Psilanthus. In Wild Crop Relatives: Genomic and Breeding Resources, Plantation and Ornamental Crops. Edited by C Kole, (2011); pp; 41–60. Berlin: Springer-Verlag.

Lashermes P, Combes MC, Robert J, Trouslot P, D’Hont A , et al . Molecular characterization and origin of the Coffea arabica L. genome. Molecular and General Genetics MGG, (1999); 261:259–266.

Tounekti T, Mahdhi M, Al-Turki TA, Khemira H. Genetic Diversity Analysis of Coffee (Coffea arabica L.) Germplasm Accessions Growing in the Southwestern Saudi Arabia Using Quantitative Traits. Natural Resources, (2017); 8 (5): 321-336.

Chen Z J. Molecular mechanisms of polyploidy and hybrid vigor. Trends in Plant Science, (2010); 15(2):57–71.

Davis AP, Tosh J, Ruch N, Fay MF. Growing coffee: Psilanthus (Rubiaceae) subsumed on the basis of molecular and morphological data; implications for the size, morphology, distribution and evolutionary history of Coffea. Botanical Journal Linnean Society’s, (2011); 167 (4):357–377.

Charrier A, Eskes AB. Botany and genetics of coffee. In:Wintgens JN (ed) Coffee: Growing, Processing, Sustainable Production A Guidebook for Growers, Processors, Traders, and Researchers. WILEY-VCH Verlag GmbH and Co. KCaA, Weinheim, Germany. 2014

Giovanni J, Nguyen C, Ampofo B, Zhong S, Fei Z. The Epigenome and Transcriptional Dynamics of Fruit Ripening. Annual Review of Plant Biology, (2017); 68:61–84.

Wintgens J N. The Coffee Plant. In Coffee: Crowing, Processing, Sustuinuchle Production, ed. J. N. Wintgens, 1–24. Weinheim: Wiley-VCH Verlad GmbH and Co.2004.

Ashihara H, Monteiro AM, Gillies FM, Crozier A. Biosynthesis of Caffeine in Leaves of Coffee. Plant Physiolgy. (1996), 111:747-753.

Ghosh P, Venkatachalapathy N. Processing and drying of coffee a review. – International Journal of Engineering Research and Technology, (2014); 3(12): 784–794.

Esquivel P, Jiménez VM. Functional properties of coffee and coffee by-products. Food Research International, (2012); 46 (2): 488-495.

Barham E. (2003) Translating terroir: the global challenge of French AOC labelling. Journal of Rural Studies, (2003); 19:127–138.

Rodrigues CI, Maia R, Miranda M, Ribeirinho M, Nogueira JMF, et al . Stable isotope analysis for green coffee bean: a possible method for geographic origin discrimination. Journal of Food Composition and Analysis, (2009); 22(5): 463–471.

Cannell, M.G.R. Physiology of the coffee crop. In Coffee: Botany, Biochemistry and Production of Beans and Beverage. Edited by Clifford, M.N., Wilson, K, pp 108–34. London: Croom Helm.1985

Clark I, Landolt HP. Coffee, caffeine and sleep: a systematic review of epidemiological studies and randomized controlled trials. Sleep Medicine Reviews, (2017); 31: 70-78.

De Mejia EG, Ramirez-mares MV. The impact of caffeine and coffee on our health. Trends in Endocrinology and Metabolism, (2014); 25(10): 489-492.

Grosso G, Micek A, Godos J, Sciacca S, Pajak A, et al. Coffee consumption and risk of all-cause, cardiovascular, and cancer mortality in smokers and non-smokers: a dose response meta-analysis. European Journal of Epidemiology, (2016); 31: 1191–1205.

Nawrot P, Jordan S, Eastwood J, Rotstein J, Hugenholtz A, Feeley M. Effects of caffeine on human health. Food Additives and Contaminants, (2003); 20 (1):1-30.

Higdon, J.V., Frei, B. Coffee and health: a review of recent human research. Critical Reviews in Food Science and Nutrition, (2006); 46(2): 101-123.

Wachamo HL. Review on Health Benefit and Risk of Coffee ConsumptionWachamo. Medicinal and Aromatic Plants, (2017); 6(4): 2-12.

Casal S, Rebelo I. Coffee: A Dietary intervention on type 2 diabetes? Current Medicinal Chemistry, (2017); 24: 376-383.

Kale LB, Reddy KJ. A study on caffeine consumption and its association with stress and appetite among call centre employees in Mumbai city, India. International Journal of Community Medicine and Public Health, (2017); 4(3):835-840.

Tajik N, Tajik M, Mack I, Enck P. The potential effects of chlorogenic acid, the main phenolic components in coffee, on health: acomprehensive review of the literature. European Journal of Nutrition, (2017); 56: 2215–2244.

Ranheim T, Halvorsen B. Coffee consumption and human health beneficial or detrimental? Mechanisms for effects of coffee consumption on different risk factors for cardiovascular disease and type 2 diabetes mellitus. Molecular Nutrition and Food Research, (2005); 49: 274-284.

Arab L. Epidemiologic evidence on coffee and cancer. Nutrition and Cancer, (2010); 62:271-283.

Cavin C, Holzhaeuser D, Scharf G, Constable A, Huber W, et al. Cafestol and kahweol, two coffee specific diterpenes with anticarcinogenic activity. Food and Chemical Toxicology, (2002); 40(8): 1155-1163.

Post SM, De wit EC, Princen HM. Cafestol, the cholesterol-raising factor in boiled coffee, suppresses bile acid synthesis by downregulation of cholesterol 7α-hydroxylase and sterol 27-hydroxylase in rat hepatocytes. Arteriosclerosis, Thrombosis and Vascular Biology, (1997); 17(11): 3064–3070.

Campa C, Mondolot L, Rakotondravao A, Bidel LPR, Gargadennec A, et al. A survey of mangiferin and hydroxycinnamic acid ester accumulation in coffee (Coffea) leaves: biological implications and uses. Annals of Botany, (2012); 110(3): 595-613.

Júnior APD, Shimizu MM, Moura JCMSM, Catharino RR, Ramos RA, et al. Looking for the physiological role of anthocyanins in the leaves of coffea arabica. Photochemistry and Photobiology, (2012); 88(4): 928–937.

Patay E B, Nemeth T, Nemeth T S, Filep R, Vlase L, Papp N. Histological and phytochemical studies of Coffea benghalensis Roxb. Ex. Schult. compared with Coffea arabica L. Farmacia. Farmacia , (2016) ; 64(1): 125–130.

Ross I A. Medicinal plants of the world. New Jersey: Humana Press. 2005.

Mullen W, Nemzer B, Ou B, Stalmach A, Hunter J, et al . The antioxidant and chlorogenic acid profiles of whole coffee fruits are influenced by the extraction procedures. Journal of Agricultural and Food Chemistry, (2001); 59(8): 3754–3762.

Johnston KL, Clifford MN, Morgan LM. Coffee acutely modifies gastrointestinal hormone secretion and glucose tolerance in humans: glycemic effects of chlorogenic acid and caffeine. The American Journal of Clinical Nutrition, (2003); 78(4): 728–733.

Tyszka-Czochara M, Pawel K, Marcin M. Caffeic acid expands anti-tumor effect of metformin in human metastatic cervical carcinoma HTB-34 cells: implications of AMPK activation and impairment of fatty acids de novo biosynthesis. International Journal of Molecular Sciences, (2017); 18 (2): 462-474.

Emura M, Nohara I, Toyoda T, Kanisawa T. The volatile constituents of the coffee flower (Coffea arabica L.). Flavour and Fragrance Journal, (1997); 12: 9-13.

Ferrari M, Ravera F, De Angelis E, Suggiliverani F, Navarini L. Interfacial prop-erties of coffee oil . Colloids and surfaces A: Physical chemical and engineering aspects, (2010); 365(1-3): 79–82.

Schroth G, Laderach P, Dempewolf J, Philpott S, Haggar J, et al. Towards a climate change adaptation strategy for coffee communities and ecosystems in the Sierra Madre de Chiapas, Mexico. Mitigation and Adaptation Strategies for Global Change, (2009); 14: 605–625.

Rivelli D P, Ropke C D, Silva V V, Miranda D V, Almeida R L, et al . Simultaneous determination of chlorogenic acid, caffeic acid and caffeine in hydroalcoholic and aqueous extracts of Ilex paraguariensis by HPLC and correlation with antioxidant capacity of the extracts by DPPH· reduction. Revista Brasileira de Ciências Farmacêuticas, (2007); 43(2): 215-222.

Parras P, Martínez-Tomé M, Jiménez AM, Murcia M A. Antioxidant capacity of coffees of several origins brewed following three different procedures. Food Chemistry, (2007); 102(3): 582-592.

Wagemaker TAL, Carvalho CRL, Maia NB, Baggio SR, Filho GO. Sun protection factor, content and composition of lipid fraction of green cof¬fee beans. Industrial Crops and Products, (2011); 33(2): 469–473.

Martín MJ, Pablos F, González AG, Valdenebro MS, León-Camacho M. Fatty acid profiles as discriminant parameters for coffee varieties dif¬ferentiation. Talanta, (2001); 54(2): 291–297.

Calligaris S, Mundari M, Gianmichele A, Barba L. Insights into the physicochemical properties of coffee oil. European Journal of Lipid Science and Technology, (2009); 111:1270–1277.

Nurman S, Lamona A, Nasir M. Optimization and characterization of n-hexane extracts of arabica coffee ground (Coffea arabica L.) fromgayo plateau as source of natural antioxidant. Journal of Physics: Conference Series, (2019); 1232: 012049.

Ivamoto ST, Sakuray, LM Ferreira LP, Kitzberger, CSG, et al. Diterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development. Plant Physiology and Biochemistry, (2016); 111:340-347.

Speer K, Kölling-Speer. Lipids In: Coffee Recent Developments. Oxford, Blackwell Science, (2001); 33-49.

Reverchon E, De Marco I. Supercritical fluid extraction and fractionation of natural matter. Journal of Supercritical Fluids, (2006); 38 (2): 146–166.

Barbosa HMA, Melo MMR, Coimbra MA, Passos CP, Silva CM. Optimiza-tion of the supercritical fluid coextraction of oil and diterpenes from spentcoffee grounds using experimental design and response surface methodology. The Journal of Supercritical Fluids, (2014); 85: 165–172.

Ahangari B, Sargolzaei J. Extraction of lipids from spent coffee grounds using organic solvents and supercritical carbon dioxide. Journal of Food Processing and Preservation, (2013); 37:1014–1021.

Pourmortazavi SM, Hajimirsadeghi SS. Supercritical fluid extraction in plant essential and volatile oil analysis. Journal of Chromatography, (2007); 1163(1-2): 2–24.

Couto RM, Fernandes J, da Silva MDRG, Simões PC. Supercritical fluid extraction of lipids from spent coffee grounds. Journal of Supercritical Fluids, (2009); 51: 159–166.

Andrade KS, Gonçalvez RT, Maraschin M, Ribeiro-Do-Valle RM, Martnez J, Ferreira SRS.Supercritical fluid extraction from spent coffee grounds andcoffee husks: antioxidant activity and effect of operational variables on extract composition.Talanta, (2012); 88: 544–552.

Oliveira NA, Santiago HPC, Fukumasu H, Oliveira AL. Green coffee extracts rich in diterpenes e process optimization of pressurized liquid extraction using ethanol as solvent. Journal of Food Engineering, (2018); 224: 148–155.

Jessop PG, Subramaniam B. Gas-expanded liquids. Chemical Reviews,(2007) 107(6): 2666–2694.

Herrero M, Mendiola JA, Ibanez E, Turner C, Wang J. Gas expanded liquids and switchable solvents. Current Opinion in Green and Sustainable Chemistry, (2017); 5:24–30.

Siougkrou E, Galindo A, Adjiman CS. On the optimal design of gas-expanded liquids based on process performance. Chemical Engineering Science, (2014); 115, 19–30.

Rodrigues PC, Mendiola JA, Quirantes-Piné PR, Ibanez E, Segura CA. Green downstream processing using supercritical carbon dioxide, CO 2 -expanded ethanol and pressurized hot water extractions for recovering bioactive compounds from Moringa oleifera leaves. Journal of Supercritical Fluids, (2016); 116: 90–100.

Toda TA, Sawada MM, Rodrigues CEC. Kinetics of soybean oil extractionusing ethanol as solvent: experimental data and modeling. Food and Bioproducts Processing, (2016); 98, 1–10.

Dórea JG, Da costa THM. Is coffee a functional food? British Journal of Nutrition, (2005); 93 (6): 773-782.

Loader TB, Taylor CG, Zahradka P, Jones P J. Chlorogenic acid from coffee beans: evaluating the evidence for a blood pressure-regulating health claim. Nutrition Reviews. (2017); 75(2): 114-133.

Leitzmann MF, Stampfer MJ, Willett WC, Spiegelman D, Colditz GA, et al . Coffee intake is associated with lower risk of symptomatic gallstone disease in women. Gastroenterology, (2002); 123(6): 1823-1830.

O'keefe JH, Bhatti SK, Patil HR, Dinicolantonio, JJ, Lucan SC, et al. Effects of habitual coffee consumption on cardiometabolic disease, cardiovascular health, and all-cause mortality. Journal of the American College of Cardiology, (2013); 62(12): 1043-1051.

Van dam RM, Willett WC, Manson JE, Hu FB. Coffee, caffeine, and risk of type 2 diabetes. Diabetes Care. (2006), 29(2): 398-403.

Martınez-Tome M, Jimenez-Monreal AM, Garcıa-Jimenez L, Almela L, Garcıa-Diz, L, et al. Assessment of antimicrobial activity of coffee brewed in three different ways from different origins. European Food Research and Technology, (2011); 233:497–505.

Nassar O M, El-Sayed M H, Kobisi A A. Estimation of Total Phenolic Contents and In vitro Antioxidant and Antimicrobial Activities of the Most Common Coffee Brews Available in the Local Markets of the Northern Region of Saudi Arabia . Journal of Pharmaceutical Research International, (2019); 31(1): 1-8.

Dogasaki C, Shindo T, Furuhata K, Fukuyama M. Identification of chemical structure of antibacterial components against Legionella pneumophilia in a coffee beverage. Yakugaku Zasshi. (2002); 122(7):487-494.

Je Y, Liu W, Giovannucci E. Coffee consumption and risk of colorectal cancer: a systematic review and meta‐analysis of prospective cohort studies. International Journal of Cancer, (2009); 124:1662-1668.

Nkondjock, A. Coffee consumption and the risk of cancer: an overview. Cancer Letters, (2009); 277 (2): 121-125.

Saab S, Mallam D, Cox GA, Ton, MJ. The impact of coffee on liver diseases: a systematic review. Liver International. (2014); 34: 495-504.

Gan Y, Wu J, Zhang S, Li L, Cao S, et al. Association of coffee consumption with risk of colorectal cancer: a meta-analysis of prospective cohort studies. Oncotarget, (2017); 8(12): 18699–18711.

Bułdak R J, Hejmo T , Osowski M , Bułdak L , Kukla M , Polaniak R , Birkner E. The impact of coffee and its selected bioactive compounds on the development and progression of colorectal cancer in vivo and in vitro. Molecules,(2018); 23(12): 3309-3333.

Brezová V, Šlebodová A, Staško A. Coffee as a source of antioxidants: An EPR study. Food Chemistry, (2009); 114(3): 859-868.

Olthof MR, Hollman, PC, Katan, MB. Chlorogenic acid and caffeic acid are absorbed in humans. Journal of Nutrition, (2001); 131(1): 66-71.

Palupi N W, Praptiningsih Y. Oxidized rapioca starch as an alginate substitute for encapsulation of antioxidant from coffee residue. Agriculture and Agricultural Science Procedia, (2016); 9:304-308.

Xu H, Wang W, Liu X, Yuan F, Gao Y. Antioxidative phenolics obtained from spent coffee grounds (Coffea arabica L.) by subcritical water extraction. Industrial Crops and Products, (2015); 76: 946–954.

Sant’Anna V, Biondo E, Kolchinski EM, Silva LFS, Correa APF, Bach E, Brandelli A. Total Polyphenols, Antioxidant, Antimicrobial and Allelopathic Activities of Spend Coffee Ground Aqueous Extract. Waste Biomass Valorization, (2017); 8: 439–442.

Namba T, Matuse T. A historical study of coffee in Japanese and Asian countries: Focusing the medicinal uses in Asian traditional medicines. Yakushigaku Zasshi, (2002); 37 (1): 65-75.

Loy V. Health maintenance in liver disease and cirrhosis. Liver Disorders, (2017); pp 89-98.

Prieto MA, Vazquez JA. In vitro determination of the lipophilic and hydrophilic antioxidant capacity of unroasted coffee bean extracts and their synergistic and antagonistic effects. Food Research International, (2014); 62:1183-1196.

Naidu MM, Sulochanamma G, Sampathu SR, Srinivas P. Studies on extraction and antioxidant potential of green coffee. Food Chemistry, (2008); 107(1): 377-384.

Kiattisin K, Nitthikan N, Poomanee W, Leelapornpisid P, Viernstein H, et al . Anti-inflammatory, antioxidant activities and safety of Coffea arabica leaf extract for alternative cosmetic ingredient. Chiang Mai Journal of Science, (2019); 46(2): 284-294.

Wen X, Takenaka M, Murata M, Homma S. Antioxidative activity of a zinc-chelating substance in coffee. Bioscience, Biotechnology, and Biochemistry, (2004); 68(11): 2313–2318.

Velazquez PMC, Dieamant GC, Eberlin S, Nogueira C, Colombi D, et al. Effect of green Coffea arabica L. seed oil on extracellular matrix components and water-channel expression in in vitro and ex vivo human skin models.- Journal of Cosmetic Dermatology, (2009); 8(1): 56-62.

Yang C, Hung F, Chen B. Preparation of coffee oil-algae oil-based nanoemulsions and the study of their inhibition effect on UVA-induced skin damage in mice and melanoma cell growth. International Journal of Nanomedicine, (2017); 12: 6559–6580.

Choi SY, Lee KJ, Kim HG, Han EH, Chung YC, et al. Protective effect of the coffee diterpenes kahweol and cafestol on tert-butyl hydroperoxide-induced oxidative hapatotoxicity. Bulletin of the Korean Chemical Society, (2016); 27 (9):1386-1392.

Wagemaker TA, Silva SA, Leonardi GR, Campos PMM. Green Coffee arabica L. seed oil influences the stability and protective effects of topical formulations. Industrial Crops and Products, (2015); 63:34–40.

Cheng B, Furtado A, Henry R J. The coffee bean transcriptome explains the accumulation of the major bean components through ripening. Scientific Reports, (2018); (8): 11414.

ICO (International Coffee Organization). International Coffee Organization trade statistics. (2016). Retrieved from.

Oliveira PMA, Almeida RH, Oliveira NA, Bostyn S. Enrichment of diterpenes in green coffee oil using supercritical fluid extraction-characterization and comparison with green coffee oil from pressing. Journal of Supercritical Fluids, (2014); 95: 137–145.

AL-Asmari KM, Zeid IMA, Al-Attar AM. Coffee Arabica in Saudi Arabia: An Overview. International Journal of Pharmaceutical and Phytopharmacological Research (eIJPPR), (2020); 10(4): 71-78.‏‏

Mak YW, Chuah L O, Ahmad R, Bhat, R. Antioxidant and antibacterial activities of hibiscus (Hibiscus rosa-sinensis L.) and Cassia (Senna bicapsularis L.) flower extracts. Journal of King Saud University-Science, (2013); 25(4): 275-282.


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