Morphological and biochemical characterization of Xanthomonas oryzae pv. oryzae isolates collected from Punjab during 2013

Hafiz Muhammad Imran Arshad, Saima Naureen, Kamran Saleem, Safdar Ali, Tanzila Jabeen, Muhammad Masood Babar


Background: Bacterial leaf blight (BLB) of rice occupies the most significant position among various potentially important bacterial diseases all over Pakistan. The first important step towards the management of this disease is the characterization of the pathogen.

Methods: Survey was conducted and disease samples were collected from 9 different locations of Punjab. Isolation and characterization by biochemical tests was done from the diseased samples under standard conditions in the laboratory. For pathogenicity and virulence characterization, all isolates were inoculated on IR-24, Basmati super and Basmati-2000 varieties. Data was recorded after three weeks of inoculation.

Results: 30 isolates of Xanthomonas oryzae pv. oryzae (Xoo) were purified from the diseased plants of different geographical origin which had very similar morphological and biochemical characteristics. PXo-20 (causing 51.35% leaf damage) and PXo-16 (causing 50.05% leaf damage) were found most virulent for Basmati Super and Basmati-2000.

Conclusion: The presence of highly virulent isolates in Sheikhupura is alarming and there is dire need to incorporate new resistance genes in commercial rice cultivars to cope with BLB.

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Khan AS, Imran M, Ashfaq M. Estimation of genetic variability and correlation for grain yield components in rice (Oryza sativa L.). Journal of Agriculture and Environtal Sciences, (2009); 6585-590.

Naveed SA, Babar M, Arif A, Zafar Y, Sabar M, et al. Detection of bacterial blight resistant gene xa5 using linked marker approaches. African Journal of Biotechnology, (2010); 9(24): 3549-3554.

Swings J, Van den Mooter M, Vauterin L, Hoste B, Gillis M, et al. Reclassification of the Causal Agents of Bacterial Blight (Xanthomonas campestris pv. oryzae) and Bacterial Leaf Streak (Xanthomonas campestris pv. oryzicola) of Rice as Pathovars of Xanthomonas oryzae (ex Ishiyama 1922) sp. nov., nom. rev. International Journal of Systematic Bacteriology, (1990); 40(3): 309-311.

Onasanya A, Ekperigin M, Nwilene F, Sere Y, Onasanya R. Two pathotypes of Xanthomonas oryzae pv. oryzae virulence identified in West Africa. Current Research in Bacteriology, (2009); 2(2): 22-35.

Khan J, Jamil F, Gill M. Screening of rice varieties/lines against bakanae and bacterial leaf blight (BLB). Pakistan Journal of Phytopathology, (2000); 12(1): 6-11.

Khan MA, Naeem M, Iqbal M. Breeding approaches for bacterial leaf blight resistance in rice (Oryza sativa L.), current status and future directions. European Journal of Plant Pathology, (2014); 139(1): 27-37.

Wang Y, Pi L, Chen X, Chakrabarty P, Jiang J. The ubiquitin ligase XB3 interacts with the rice receptor-like kinase Xa21 and is required for Xa-21 mediated disease resistance. Plant Cell, (2006); 18: 3635-3646.

Dinh HD, Oanh NK, Toan ND, Van Du P, Loan LC. Pathotype profile of Xanthomonas oryzae pv oryzae isolates from the rice ecosystem in Cuulong Rever Delta. Omonrice, (2008); 16(1): 34-40.

Udayashankar A, Nayaka SC, Reddy M, Srinivas C. Plant growth-promoting rhizobacteria mediate induced systemic resistance in rice against bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae. Biological Control, (2011); 59(2): 114-122.

Jabeen R, Ashraf M, Ahmad I. Evaluating the effects of cold water diffusates against Xanthomonas oryzae pv. oryzae causing bacterial leaf blight (BLB) in rice. Archives of Phytopathology and Plant Protection, (2009); 42(2): 173-187.

Noda T, Li C, Li J, Ochiai H, Ise K, et al. Pathogenic diversity of Xanthomonas oryzae pv. oryzae strains from Yunnan province, China. Japan Agricultural Research Quarterly, (2001); 35(2): 97-104.

khtar MA, Abbasi FM, Ahmad F, Shahzad M, Shah MA, et al. Evaluation of rice germplasm againt Xanthomonas oryzae causing bacterial leaf blight. Pakistan Journal of Botany, (2011); 43(6): 3021-3023.

Jabeen R. Medicinal plants-a potent antibacterial source against bacterial leaf blight (BLB) of rice. Pakistan Journal Botany (2011); 43111-118.

Ali A, Khan MH, Bano R, Rashid H, Raja NI, et al. Screening of Pakistani rice (Oryzae sativa) cultivars against Xanthomonas oryza pv oryzae. Pakistan Journal of Botany, (2009); 41(5): 2595-2604.

Akhtar M, Rafi A, Hamed A. Comparison of methods of inoculation of Xanthomonas oryzae pv. oryzae in rice cultivar. Pakistan Journal of Botany, (2008); 40(5): 2171-2175.

Mew T. Current status and future prospects of research on bacterial blight of rice. Annual Review of Phytopathology, (1987); 25(1): 359-382.

Gnanamanickam S, Priyadarisini VB, Narayanan N, Vasudevan P, Kavitha S. An overview of bacterial blight disease of rice and strategies for its management. Current Science, (1999); 77(11): 1435-1444.

Agarwal P, Mortensen CN, Mathur S. Seed-borne diseases and seed health testing of rice. Phytopathological papers, (1989); (30).

Ding X, Cao Y, Huang L, Zhao J, Xu C, et al. Activation of the indole-3-acetic acid–amido synthetase GH3-8 suppresses expansin expression and promotes salicylate-and jasmonate-independent basal immunity in rice. The Plant Cell Online, (2008); 20(1): 228-240.

Khan JA, Arshad HMI, Saleem K, Sandhu AF, Hasnain S, et al. Evaluation of resistance genes in rice against local isolates of Xanthomonas oryzae pv. oryzae in Punjab Province of Pakistan. Archives of Phytopathology and Plant Protection, (2012); 45(15): 1826-1839.

Khan MAU, Shahid AA, Rao AQ, Kiani S, Ashraf MA, et al. Role of epicuticular waxes in the susceptibility of cotton leaf curl virus (CLCuV). African Journal of Biotechnology, (2013); 10(77): 17868-17874.

Muzaffar A, Kiani S, Khan MAU, Rao AQ, Ali A, et al. Chloroplast localization of Cry1Ac and Cry2A protein-an alternative way of insect control in cotton. Biological Research, (2015); 481-11.

Awan M, Abass M, Muzaffar A, Ali A, Tabassum B, et al. Transformation of Insect and Herbicide Resistance Genes in Cotton (Gossypium hirsutum L.). Journal of Agricultural Science and Technology, (2015); 17(2): 287-298.


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