In vitro step up NaCl treatment method as inducer of salinity tolerance in Basmati rice varieties
Abstract
Background: It has been recorded that salt negatively impacts rice crop's seedling and reproductive stages. Globally, soil salinity is 2nd most important abiotic factor, profoundly impacting rice productivity. The purpose of current research is to persuade tolerance for salinity in salt sensitive varieties of Basmati rice [Basmati-385 (Bas-385), Super Basmati (Sup-Bas) and Basmati-2000 (Bas-2000)] by use of in vitro step-up NaCl treatment method.
Methods: The in vitro step up NaCl treatment method was used to produce salt-tolerant calli from salt-sensitive Basmati rice varieties. The regeneration of these salt-tolerant calli in a salt-free medium led to the M1 generation. Subsequently, M2 and M3 generations were cultivated and garnered under salt stress conditions.
Results: The highest callus induction percentage was achieved for all Basmati rice varieties by using MS medium supplemented with 2.0 mg/L 2,4-D. These calli underwent in vitro step up NaCl treatments, with the maximum tolerance level to NaCl being 1.0% across all varieties. Bas-385 and Bas-2000 exhibited regeneration frequencies of 75% and 85% respectively when cultured in MS medium supplemented with 2.0 mg/L BAP and 0.5 mg/L NAA, while Sup-Bas displayed a regeneration frequency of 79% in MS medium comprising 3.0 mg/L BAP with 0.5 mg/L NAA. In subsequent M2 and M3 generations, a decline was observed in all selected agronomic and morphological features in all varieties under salinity stress in comparison to parent plants.
Conclusion: This research validates the induction of salt tolerance in salt susceptible varieties of Basmati rice by in vitro step up NaCl treatment technique.
Keywords: In vitro selection; Salt tolerance; Basmati rice; Step up NaCl treatment; Callogenesis; Plant regeneration
Full Text:
PDFReferences
Joshi RK, Rao GJN. Somaclonal variation in submergence tolerant rice cultivars and induced diversity evaluation by PCR markers. International Journal of Genetics and Molecular Biology, (2009); 1(5): 80-88.
Shitu GA, Nain MS, Singh R. Developing extension model for uptake of Precision conservation agricultural practices in developing nations: learning from rice–wheat system of Africa and India. Current Science, (2018); 114(4): 814-825.
FAOSTAT: Food and Agriculture Organization of the United Nations. (2020). Production/Yield Quantities of Rice, paddy in World. http://www.fao.org/faostat/en/#data/QC/visualize
Shahbandeh M. World rice acreage 2010-2018. (2020). https://www.statista.com/statistics/271969/world-rice-acreage-since-2008/
Chauhan BS, Jabran K, Mahajan G. (2017). Rice Production Worldwide. (2017); Springer International Publishing AG, Switzerland.
Economic Survey of Pakistan 2019–2020. (2020).
http://www.finance.gov.pk/survey/chapter_20/02_Agriculture.pdf
Mahmood MA, Sheikh AD, Kashif M. Acreage supply response of rice in Punjab, Pakistan. Journal of Agricultural Research, (2007); 45(3): 231-236.
Grover A, Minhas D. Towards production of abiotic stress tolerant transgenic rice plants: issues, progress and future research eeds. In: Proc: Indian National Science Academy. Part B. Reviews and Tracts. Biological Sciences, (2000); 66 (1): 13-32.
Ansari MR, Shaheen T, Bukhari SA, Husnain, T. Genetic improvement of rice for biotic and abiotic stress tolerance. Turkish Journal of Botany, (2015); 39: 911-919.
Fogliatto S, Serra F, Patrucco L, Milan M, Vidotto F. Effect of different water salinity levels on the germination of lmazamox-resistant and sensitive weedy rice and cultivated rice. Agronomy, (2019); 9: 658-670.
IRRI. (1997). Screening Rice for Salinity Tolerance. G.B. Gregorio, D. Senadhira & R.D. Mendoza. IRRI Discussion Paper Series No. 22.
IRRI. (2006). Bringing Hope, Improving Lives: Strategic Plan 2007–2015. Manila.p 61.
Acosta-Motos JR, Ortuno M, Bernal-Vicente, A., Diaz-Vivancos P, Sanchez-Blanco MJ. Hernandez JA. Plant responses to salt stress: adaptive mechanisms. Agronomy, (2017); 7: 18.
Khan MA, Abdullah Z. Salinity-sodicity induced changes in reproductive physiology of rice (Oryza sativa) under dense soil conditions. Environmental and Experimental Botany, (2003); 49: 145-157.
Cha-um S, Trakulyingcharoen T, Smitamana P, Kirdmanee C. Salt tolerance in two rice cultivars differing in salt tolerant abilities in responses to Iso-osmotic stress. Australian Journal of Crop Science, (2009); 3(4): 221-230.
Qureshi RH, Aslam M, Mustafa G, Akhtar J. Some aspects of physiology of salt tolerance in wheat (Triticum aestivum L). Pakistan Journal of Agricultural Sciences, (1991); 28: 199-209.
Aslam M, Ahmad I, Mahmood IA, Akhtar J, Nawaz S. Physiological basis of differential tolerance in rice to Salinity. Pakistan Journal of Soil Sciences, (1995); 10: 38-41.
Shereen A, Mumtaz S, Raza S, Khan MA, Solangi S. Salinity effects on seedling growth and yield components of different inbred rice lines. Pakistan Journal of Botany, (2005); 37(1): 131-139.
Agricultural Statistics of Pakistan. (2019-20). Government of Pakistan, Ministry of Food, Agriculture and Livestock, Economic Wing, Islamabad.
Samal P, Babu S. The Shape of Rice Agriculture towards 2050. International Association of Agricultural Economists (IAAE), (2018); Conference, July 28-August 2, 2018, Vancouver, British Columbia.
https://ageconsearch.umn.edu/record/277550
Nikam AA, Devarumath RM, Ahuja A, Babu H, Shitole MG, Suprasanna P. Radiation-induced in vitro mutagenesis system for salt tolerance and other agronomic characters in sugarcane (Saccharum officinarum L.). The Crop Journal, (2015); 3: 46-56.
Miki Y, Hashiba M, Hisajima S. Establishment of salt stress tolerant rice plants through step up NaCl treatment in vitro. Biologia Plantarum, (2001a); 44(3): 391-395.
Miki Y, Katoh M, Hisajima S. Salt tolerance of in vitro established salt-tolerant rice plants during further growth in soil. Biologia Plantarum, (2001b); 44(3): 463-466.
Tam DM, Lang NT. In vitro selection for salt tolerance in rice. Omonrice, (2003); 11: 68-73.
Sharry SE, Silva JAT. Effective organogenesis, somatic embryogenesis, and salt tolerance induction in vitro in the Persian Lilac tree (Melia azedarach L.). Floriculture, Ornamental and Plant Biotechnology, (2006); 2: 317-324.
Mahmood A, Latif T, Khan MA. Effect of salinity on growth, yield and yield components in Basmati rice germplasm. Pakistan Journal of Botany, (2009); 41(6): 3035-3045.
Murashige T, Skoog F. A revised medium for rapid growth and bioassay with Tobacco tissue cultures. Physiologia Plantarum, (1962); 15: 473-497.
Gupta B, Huang B. Mechanism of salinity tolerance in plants: Physiological, biochemical and molecular characterization. International Journal of Genomics, (2014); Article ID 701596, 18. https://doi.org/10.1155/2014/701596
Ashraf M. Some important physiological selection criteria for salt tolerance in plants. Flora, (2004); 199: 361–376.
Rai MK, Kaliaa RK, SinghaR, Gangolaa MP, Dhawana, AK. Developing stress tolerant plants through in vitro selection—An overview of the recent progress. Environmental and Experimental Botany, (2010); 71: 89–98
Ullah H, Ullah I, Jadoon SA, Rashid H. Tissue culture techniques for callus induction in rice. Sarhad Journal of Agriculture, (2007); 23(1): 81-86.
Afrasiab H, Jafar R. Effect of different media and solidifying agents on callogenesis and plant regeneration from different explants of rice (Oryza sativa L.) varieties Super Basmati and IRRI-6. Pakistan Journal of Botany, (2011); 43(1): 487-501.
Rafique MZ, Rashid H, Chaudhary MF, Chaudhry Z, Cheema NM. Study on callogenesis and organogenesis in local cultivars of rice (Oryza sativa L.). Pakistan Journal of Botany, (2011); 43(1): 191-203.
Singh S, Kumar A, Rana V, Kumar A, Sharma VR. In Vitro callus induction and plant regeneration in basmati rice (Oryza sativa L.) varieties. Advances in Agriculture and Natural Sciences for Sustainable. Journal of Pharmacognosy and Phytochemistry, (2018); SP5: 65-69
Mostafiz SB, Wagiran A. Efficient callus induction and regeneration in selected Indica rice. Agronomy, 2018; 8:77
Revathi S, Pillai MA. In vitro callus induction in rice (Oryza sativa L.). Research in Plant Biology, (2011); 1:13-15.
Sikder MBH, Sen PK, Mamun MA, Ali MR, Rahman SM. In Vitro Regeneration of Aromatic Rice (Oryza sativa L.). International Journal of Agricultural and Biology, (2006); 6:759–762.
Ge XJ, Chu JH, Lin YJ, Wang SP. A tissue culture system for different germplasms of indica rice. Plant Cell Reports, (2006); 25:392-402.
Upadhyaya G, Sen M, Roy A. In vitro callus induction and plant regeneration of rice (Oryza sativa L.) var. ‘Sita’, ‘Rupali’ and ‘Swarna Masuri”. Asian Journal of Plant Science and Research, (2015); 5:24-27.
Htwe NN, Maziah M, Ling HC, Zaman, FQ, Zain AM. Responses of some selected
Malaysian rice genotypes to callus induction under in vitro salt stress. African Journal of
Biotechnology, (2011);10: 350-362.
Wu J, Seliskar DM, Gallagher JL. The response of plasma membrane lipid composition in callus of the halophyte Spartina patens (Poaceae) to salinity stress. American Journal of Botany, (2005); 92: 852-858.
Queiros F, Fidalgo, F, Santos I, Salem R. In vitro selection of salt tolerant cell lines in Solanum tuberosum L. Biologia Plantarum, (2007); 51(4): 728–734.
Khorami A, Safarnejad A. 2011. In vitro selection of Foeniculum vulgare for salt tolerance. Notulae Scientia Biologicae, (2011); 3: 90-97.
Saffan SES. Effect of salinity and osmotic stresses on some economic plants. Research
Journal of Agriculture and Biological Sciences, (2008); 4: 159-166.
R. Munns, Tester, M. Mechanisms of salinity tolerance. Annual Review of Plant Biology, (2008); 59: 651-681.
Hakim MA, Juraimi AS, Begum M, Hanafi MM, Ismail MR, Selamat A.
Effect of salt stress on germination and early seedling growth of rice (Oryza sativa L.). African Journal of Biotechnology, (2010); 9(13): 1911-1918
Hussain S, Cao X, Zhong C, Zhu L, Khaskheli1 MA, Fiaz S, Zhang J, Jin Q. Sodium
chloride stress during early growth stages altered physiological and growth characteristics
of rice. Chilean Journal of Agricultural Research, (2018); 78(2): 183-197
Verslues PE, Agarwal M, Katiyar-Agarwal S, Zhu JH, Zhu, JK. Methods and concepts in
quantifying resistance to drought, salt and freezing, abiotic stresses that affect plant
water status. The Plant Journal, (2006); 45(4): 523-539.
Khatun S, Flowers, TJ. Effects of salinity on seed set in rice. Plant Cell Environment, (1995); 18(1):61-67.
Singh, RK, Mishra B, Singh KN. Salt tolerant rice varieties and their role in reclamation
programme in Uttar Pradesh. Indian Farming, (2004); 6-10
DOI: http://dx.doi.org/10.62940/als.v12i1.1154
Refbacks
- There are currently no refbacks.