Presence and Antibiotic Resistance of MDR Salmonella Isolates Recovered from Zea mays L. Farms Located near the Poultry Farms in Faisalabad-Pakistan

Arslan Ali, Nimra Amjad, Fatima Javed, Zain Ul Abbas, Saima Muzammil, Muhammad Zeeshan Ahmad, Sadaf Oranab, Muhammad Umar, Maria Sajid

Abstract


Background: Salmonella is the major food-borne pathogen associated with food products and causative agent of salmonellosis. Discharge of untreated wastes and leakage of poultry drainage in irrigation water might be the significant source of contamination in fields. The aim of this study was to investigate the presence of Salmonella in the rhizosphere and phyllo sphere of Zea mays L farm, following irrigation with ditch water contaminated with poultry drainage.

Methods: Total 6 maize farms in and around Faisalabad (Pakistan) were selected nearby the poultry farm area. Irrigated water, rhizosphere and leaves were analyzed for presence of Salmonella. A total of 160 samples were collected from different farms. Samples were cultivated on SS agar media and incubated at 37oC.

Results: Out of 160 samples, 39 showed positive growth for bacterial contamination. 18 samples were confirmed as Salmonella by morphological and biochemical characteristics. Our results indicated the presence of Salmonella isolates from irrigated water (n=10), from rhizosphere (n=5), from phyllo sphere (n=1) and from roots (n=2). Antibiotics susceptibility pattern of Salmonella isolates against routinely used antibiotics had indicated that 71% isolates were resistant to Tetracycline and Amikacin, and 65% resistance to Chloramphenicol. All the isolates were sensitive to Levofloxacin, Tobramycin, Cefepime, Gentamycin, Cefoxitin and Sulfamethoxale. All isolates were intermediate resistant to cefuroxime and ampicillin.

Conclusion: From obtained result it is confirmed that Salmonella spp. have been found in irrigation water mixed with poultry drainage and could be a source of Salmonella contamination to the crops located near the poultry farms.

Keywords: Rhizosphere; Zea mays L., Ditch water; Phyllo-sphere; Irrigation water 


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DOI: http://dx.doi.org/10.62940/als.v9i4.1495

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