Stressed Experimental diabetic Rats challenging with Glimepiride

Wissam Sajid Hashim, Youssef Shakuri Yasin, Emad Ayal Muter, Yousif Ahmed Khalil


Background: This study was accomplished to evaluate the anticipated effects of glimepiride on some of the hematological parameters besides the antioxidant enzymes of the alloxan-induced diabetic and healthy rats.

Methods: In this study, thirty-two adult albino male rats were adopted. The animals were set in a random manner into four groups of eight rats each. The animals of the control group were dosed orally with 5ml distilled water. The second were injected intraperitoneally with 150 mg/kg of Alloxan one time to induce diabetes. The third group were administered a daily oral dose of 5 mg/kg of Glimepiride. The fourth group were injected with alloxan in the same manner as the second group and then dosed orally with 5mg/kg of glimepiride. The above-mentioned experimental protocol has extended for one month and thereafter the planned tests were done.

Results: The results showed that diabetes induced by alloxan led to significant decline in the packed cells volume (PCV), hemoglobin concentration (Hb), platelets (PLT), red blood cell counts (RBC), glutathione (GSH), alanine aminotransferase (ALT) and alkaline phosphatase (ALP)at (p≤0.05) with significant elevation in the total white blood cells count (WBC), malondialdehyde (MDA), erythrocytes sedimentation rate (ESR) and aspartate aminotransferase (AST) comparing with those of the control group. The use of glimepiride alone to the healthy rats led to significant decline in RBC,PLT, GSH and AST with significant elevation in ESR and WBC without an effect on the PCV, Hb, MDA, ALT and ALPat (p≤0.05) comparing with those of the control group. Treatment of alloxan-induced diabetic rats with glimepiride led to significant decline in RBC, HB and PCV with significant elevation in WBC, PLT, GSH, MDA and ALT at (p≤0.05) compared with the control group.

Conclusion: We conclude that glimepiride does affect the blood parameters and the antioxidant enzymes.

Keywords: Glimepiride; Amaryl; Stress; Diabetes; Rats    

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