The prevalence of antimicrobial-producing Gram-positive bacteria in human gut: a preliminary study

Arif Luqman, Nimerta Kumari, Jongkon Saising, Aparna Viswanathan Ammanath, Nur Hidayatul Alami, Endry Nugroho Prasetyo, Enny Zulaika, Maya Shovitri, Nengah Dwianita Kuswytasari, Tri Haidar Munif, Friedrich Götz

Abstract


Background: Human gut microbiome is an excellent source for searching novel antimicrobials which is currently in need due to the raise of drug resistance bacteria. Many Gram-positive bacteria isolated from human gut have been reported to produce antimicrobial compounds but still only few studies investigating the prevalence of these bacteria in human gut.

Methods: We took stool samples from 19 adult participants (age: 20–70 years; ethnicity: European and Asian). Stool samples obtained from 7 females and 12 males. We screened for Gram-positive antimicrobial-producing bacteria from the stool samples and identified the positive ones using 16s rRNA sequencing.

Results: Here, we reported that antimicrobial-producing Gram-positive bacteria can be found in the stool samples of 6 out of 19 participants. By screening against Staphylococcus aureus USA300 and Pseudomonas aeruginosa PAO1, some isolates exhibited a different inhibition activity compared to the previously reported antimicrobial compounds.

Conclusion: Our findings showed that some strains isolated from human gut exhibits a novel antimicrobial activity which implies that there could still be novel antimicrobial compounds in human gut produced by Gram-positive bacteria.


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References


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