The prevalence of antimicrobial-producing Gram-positive bacteria in human gut: a preliminary study
Abstract
Background: Human gut microbiome is an excellent source for searching novel antimicrobials which is currently in need due to the raise of drug resistance bacteria. Many Gram-positive bacteria isolated from human gut have been reported to produce antimicrobial compounds but still only few studies investigating the prevalence of these bacteria in human gut.
Methods: We took stool samples from 19 adult participants (age: 20–70 years; ethnicity: European and Asian). Stool samples obtained from 7 females and 12 males. We screened for Gram-positive antimicrobial-producing bacteria from the stool samples and identified the positive ones using 16s rRNA sequencing.
Results: Here, we reported that antimicrobial-producing Gram-positive bacteria can be found in the stool samples of 6 out of 19 participants. By screening against Staphylococcus aureus USA300 and Pseudomonas aeruginosa PAO1, some isolates exhibited a different inhibition activity compared to the previously reported antimicrobial compounds.
Conclusion: Our findings showed that some strains isolated from human gut exhibits a novel antimicrobial activity which implies that there could still be novel antimicrobial compounds in human gut produced by Gram-positive bacteria.
Full Text:
PDFReferences
Umu OC, Oostindjer M, Pope PB, Svihus B, Egelandsdal B, et al. Potential applications of gut microbiota to control human physiology. Antonie Van Leeuwenhoek, (2013); 104(5): 609-618.
Walsh CJ, Guinane CM, O'Toole PW, Cotter PD. Beneficial modulation of the gut microbiota. FEBS Letters, (2014); 588(22): 4120-4130.
Sommer F, Backhed F. The gut microbiota–masters of host development and physiology. Nature Reviews Microbiology, (2013); 11(4): 227-238.
Ubeda C, Djukovic A, Isaac S. Roles of the intestinal microbiota in pathogen protection. Clinical and Translational Immunology, (2017); 6(2): e128.
Reinoso Webb C, Koboziev I, Furr KL, Grisham MB. Protective and pro-inflammatory roles of intestinal bacteria. Pathophysiology, (2016); 23(2): 67-80.
Campana R, van Hemert S, Baffone W. Strain-specific probiotic properties of lactic acid bacteria and their interference with human intestinal pathogens invasion. Gut Pathogens, (2017); 912.
Iacob S, Iacob DG, Luminos LM. Intestinal Microbiota as a Host Defense Mechanism to Infectious Threats. Frontiers in Microbiology, (2018); 93328.
Wang L, Zhu L, Qin S. Gut Microbiota Modulation on Intestinal Mucosal Adaptive Immunity. Journal of Immunology Research, (2019); 20194735040.
Garcia-Gutierrez E, Mayer MJ, Cotter PD, Narbad A. Gut microbiota as a source of novel antimicrobials. Gut Microbes, (2019); 10(1): 1-21.
Schleifer K-H, Krämer E. Selective Medium for Isolating Staphylococci. Zentralblatt für Bakteriologie: I Abt Originale C: Allgemeine, angewandte und ökologische Mikrobiologie, (1980); 1(3): 270-280.
Marchesi JR, Sato T, Weightman AJ, Martin TA, Fry JC, et al. Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. Applied and Environmental Microbiology, (1998); 64(2): 795-799.
Luqman A, Nega M, Nguyen MT, Ebner P, Gotz F. SadA-Expressing Staphylococci in the Human Gut Show Increased Cell Adherence and Internalization. Cell Reports, (2018); 22(2): 535-545.
Luqman A, Muttaqin MZ, Yulaipi S, Ebner P, Matsuo M, et al. Trace amines produced by skin bacteria accelerate wound healing in mice. Communications Biology, (2020); 3(1): 277.
Kamada N, Chen GY, Inohara N, Nunez G. Control of pathogens and pathobionts by the gut microbiota. Nature Immunology, (2013); 14(7): 685-690.
Kamada N, Seo SU, Chen GY, Nunez G. Role of the gut microbiota in immunity and inflammatory disease. Nature Reviews Immunology, (2013); 13(5): 321-335.
Pickard JM, Zeng MY, Caruso R, Nunez G. Gut microbiota: Role in pathogen colonization, immune responses, and inflammatory disease. Immunological Reviews, (2017); 279(1): 70-89.
Jandhyala SM, Talukdar R, Subramanyam C, Vuyyuru H, Sasikala M, et al. Role of the normal gut microbiota. World Journal of Gastroenterology, (2015); 21(29): 8787-8803.
Balla E, Dicks LM, Du Toit M, Van Der Merwe MJ, Holzapfel WH. Characterization and cloning of the genes encoding enterocin 1071A and enterocin 1071B, two antimicrobial peptides produced by Enterococcus faecalis BFE 1071. Applied and Environmental Microbiolology, (2000); 66(4): 1298-1304.
Scott KP, Antoine JM, Midtvedt T, van Hemert S. Manipulating the gut microbiota to maintain health and treat disease. Microbial Ecology in Health and Disease (2015); 2625877.
Marchesi JR, Adams DH, Fava F, Hermes GD, Hirschfield GM, et al. The gut microbiota and host health: a new clinical frontier. Gut, (2016); 65(2): 330-339.
Dajani AS, Tom MC, Law DJ. Viridins, bacteriocins of alpha-hemolytic streptococci: isolation, characterization, and partial purification. Antimicrobial Agents and Chemotherapy, (1976); 9(1): 81-88.
Collins FWJ, O'Connor PM, O'Sullivan O, Rea MC, Hill C, et al. Formicin – a novel broad-spectrum two-component lantibiotic produced by Bacillus paralicheniformis APC 1576. Microbiology, (2016); 162(9): 1662-1671.
Nakano H, Tomita F, Yamaguchi K, Nagashima M, Suzuki T. Corynecin (chloramphenicol analogs) fermentation studies: selective production of Corynecin I by Corynebacterium hydrocarboclastus grown on acetate. Biotechnology and Bioengineering, (1977); 19(7): 1009-1018.
Suzuki T, Honda H, Katsumata R. Production of Antibacterial Compounds Analogous to Chloramphenicol by a n-Paraffin-grown Bacterium. Microbiology & Fermentation Industry, (1972); 36(12): 2223-2228.
Goh HF, Philip K. Purification and Characterization of Bacteriocin Produced by Weissella confusa A3 of Dairy Origin. PLoS One, (2015); 10(10): e0140434.
Valenzuela AS, Benomar N, Abriouel H, Canamero MM, Galvez A. Isolation and identification of Enterococcus faecium from seafoods: antimicrobial resistance and production of bacteriocin-like substances. Food Microbiology, (2010); 27(7): 955-961.
Ness IF, Diep DB, Ike Y. Enterococcal Bacteriocins and Antimicrobial Proteins that Contribute to Niche Control. 2014 Feb 16. In: Gilmore MS, Clewell DB, Ike Y, Shankar N, editors. Enterococci: From Commensals to Leading Causes of Drug Resistant Infection [Internet]. Boston: Massachusetts Eye and Ear Infirmary; 2014–. PMID: 24649514.
Martin-Platero AM, Valdivia E, Ruiz-Rodriguez M, Soler JJ, Martin-Vivaldi M, et al. Characterization of antimicrobial substances produced by Enterococcus faecalis MRR 10-3, isolated from the uropygial gland of the hoopoe (Upupa epops). Applied and Environmental Microbiology, (2006); 72(6): 4245-4249.
DOI: http://dx.doi.org/10.62940/als.v10i1.1634
Refbacks
- There are currently no refbacks.