Assessment of the Safety of Pine Tree Feed Additives

Roman Lapshin, Natalia Maksimova, Victor Lipskiy, Viktor Ryzhov, Vasily Korotky, Irina Mukhina


Background: The research was focused on evaluating the safety of a pine tree energy feed additive, a product developed by Himinvest Scientific and Technical Center LLC, located in Nizhny Novgorod, Russia. The need for this study arose from the growing utilization of such feed additives in the agriculture sector and the necessity to ensure their safe usage for livestock health and welfare.

Methods: The experiments were carried out on white outbred mice and outbred rats of the Wistar line. The authors conducted a study of acute and chronic toxicity and the local irritant effect of the pine tree energy feed additive. In the experiments, the authors did not establish gender-related differences in the sensitivity of animals to the toxic effect of the preparation.

Results: As a result of the conducted studies on white outbred mice, it was found that with a single intragastric injection of the feed additive, it was a relatively harmless substance. Besides, with repeated intragastric administration of the pine tree energy feed additive to outbred rats in subtoxic doses, no significant defects of the functional state of the main organs and systems of the body were observed.

Conclusion: The findings suggest that the pine tree energy feed additive is relatively harmless, thus supporting its safe use. No significant toxicity or irritant effects were observed in the test subjects, making it a viable option for feeding purposes.

Full Text:



Shevchenko TV, Ustinova YV, Ermolaeva EO. Preparation and Light Stabilization of Natural Anthocyanin Dyes in the Presence of Fullerene for Food Products. Egyptian Journal of Chemistry, (2022); 65(11): 105–114.

Sannikova N, Kovaleva O, Shulepova O, Bocharova A, Melnikov V. Reducing the carbon footprint when using non-metallic minerals as litter in poultry farms. Revista Ingeniería UC, (2022); 29(1): 43–50.

Raketsky VA, Nametov AM, Sozinov VA, Baisakalov AA. Increasing the efficiency of the herd reproduction system by introducing innovative technologies into dairy farming in Northern Kazakhstan. Veterinary World, (2021); 14(11): 3028–3037.

Ulrikh EV, Smolovskaya OV. Mycotoxins In Fodder And Its Importance On Safety Of Feed And The Health Of Farm Animals: A Review. Online Journal of Animal and Feed Research, (2021); 11(6): 219–223.

Korotkiy VP, Ryzhov V, Turubanov AI, Roshkin VI, Bayunova EA, Prytkov YuN, Ryzhova ES. Coniferous energy additive. Patent RF 2543814. March 3, 2015; Bulletin No. 7. Federal Service for Intellectual Property of the Russian Federation.

Korotkiy VP, Gibalkina NI, Mungin VV, Buryakov NP, Buryakova MA, Bazhenova DA, Korotkiy IV, Ryzhov VA. Method for application of coniferous energy supplement in growing calves. Patent RF 2771182. April 28, 2022; Bulletin No. 13. Federal Service for Intellectual Property of the Russian Federation.

Korotkii VP, Ryzhov VA, Bogdanovich DM, Radchikov VF, Dzhumkova MV, Sonich NA, Sadykov EV. Effektivnost ispolzovaniya kormovoi fitodobavki v kormlenii telyat [The efficiency of using feed phytonutrients in feeding calves]. Zootekhnicheskaya nauka Belarusi, (2022); 57(1): 227-234.

Zaitsev VV, Bogolyubova NV, Zaitseva LM, Emelyanova IS, Korotkiy VP, Ryzhov VA. Effect of dietary supplements farmatan TM and pine tree energy on the regulation of ruminal digestion and microbiocenosis of lactating black and white cows. Basrah Journal of Agricultural Sciences, (2022); 35(2): 99-109.

Zaitsev VV, Mayorov IN, Zaitseva LM, Korotky VP, Ryzhov VA, Buryakov NP. The effect of a forest biomass supplement on morphophysiological parameters of calves. Basrah Journal of Agricultural Sciences, (2021); 34(2): 184-192.

Mironov AN (ed). Rukovodstvo po provedeniyu doklinicheskikh issledovanii lekarstvennykh sredstv [Guidelines for conducting preclinical studies of medications]. In two parts. 2012. Grif i K, Moscow.

Bures J, Buresova O, Huston JP. Materialy i osnovnye eksperimenty po izucheniyu mozga i povedeniya [Techniques and basic experiments for the study of brain and behavior]. 1991; 397. Vysshaya shkola, Moscow.

Goldberg DI, Goldberg ED. Spravochnik po gematologii [Handbook of hematology]. 1968; 270. Izd-vo Tomskogo universiteta, Tomsk.

Gorizontov PD, Belousova ON, Fedotova MI. Stress i sistema krovi [Stress and the blood system]. 1983; 240. Meditsina, Moscow.

Menshikov ММ (ed). Klinicheskaya laboratornaya analitika [Clinical laboratory analytics]. 2003; 816. Agat-Med, Moscow.

Sarkisov DS, Perova YuL (eds). Mikroskopicheskaya tekhnika [Microscopical technique]. 1996; 544. Meditsina, Moscow.

Gayirbegov D, Engurazov G. Effect of the Cresacin Feed Additive on the Digestibility of Nutrients, Productivity and Egg Quality in Egg Laying Quails. American Journal of Animal and Veterinary Sciences, (2023); 18(1): 52-57.

Szurpnicka A, Wrońska AK, Bus K, Kozińska A, Jabłczyńska R, Szterk A, Lubelska K. Phytochemical screening and effect of Viscum album L. on monoamine oxidase A and B activity and serotonin, dopamine and serotonin receptor 5-HTR1A levels in Galleria mellonealla (Lepidoptera). Journal of Ethnopharmacology, (2022); 298.

Biehl J, Sandén H, Rewald B. Contrasting Effects of Two Hydrogels on Biomass Allocation, Needle Loss, and Root Growth of Picea Abies Seedlings Under

Drought. SSRN Electronic Journal, (2023).

Beili N. Statisticheskie metody v biologii [Statistic methods in biology]. 1963; 241. Mir, Moscow.

Kolesnik YuN, Yurina NA, Danilova AA. Phytoenergy supplement in the diet of high-producing cow. Taurida herald of the agrarian sciences, (2018); 3(15): 55-64.

Korotkiy VP, Ryzhov VA, Buryakov NP, Yurina NA, Kolesnik YN. Use of natural phytonutrients in feeding of high-producing cows. Collection of scientific papers of the Krasnodar Scientific Center for Animal Science and Veterinary Medicine, (2018); 7(1): 271-276.

Lefler TF, Murzina TV, Kirienko NN, Turitsyna EG, Rabimov AI. The influence of coniferous and energy supplements on the cows’ milk productivity.Vestnik of the Krasnoyarsk State Agrarian University, (2020); 11(164): 114-121.

Mishurov AV, Bogolyubova NV, Romanov VN, Korotkiy VP, Ryzhov VA. Complex of additional energetic feeding rations of cows. Agroindustrial Complex of Upper Volga Region Herald, (2017); 4(40): 35–38.

Bayazitova ZE, Kurmanbayeva AS, Tleuova ZO, Temirbekova NG. Application of the Thermophilic Fermentation Method to Obtain Environmentally Friendly Organic Fertilizer. Journal of Ecological Engineering, (2023); 24(4): 202-216.

Suraganova AM, Memeshov SK, Aitbayev TE, Kirchev H, Suraganov MN. Effect of Bio-Organic-Mineral Fertilizers on Quality, Yield and Safety Indicators of Potatoes under Conditions of Akmola Region. OnLine Journal of Biological Sciences, (2022); 22(1), 1-9.

Al Dhanhani ASSJ, Mirza SB, Alhefeiti FRMO, Ridouane FL. Investigating the nutritional potential and vegetation blueprint mapping of Acacia tortilis and Acacia ehrenbergiana from the origin of Fujairah, UAE, for Arabian Tahr as native fodder plants. Adv. Life Sci., (2023); 10(1): 99-103.

Ruin VA, Kistina AA, Prytkov YN. Use of the Bioprimum Sukhoy Feed Additive in Cow Feeding. J Biochem Technol. 2022;13(1):41-4.

Khayyatnezhad M, Keynoos H. Incorporating Conditional Uncertainty into Decision-making for Forecasting Actual Evapotranspiration in Semiarid Area. Adv. Life Sci., (2022); 9(4): 498-503.

Sherimova SK, Sarsembayeva NB, Abdigaliyeva TB, Lozowicka B. Vermikom feed additive effects on dairy cows' blood and milk parameters. Veterinary World, (2022); 15(5): 1228-1236.

Kenebayev AT, Meiirman GT, Yerzhanova ST, Yesimbekova MA, Abayev SS. Manifestation of Valuable Selective Traits in Alfalfa Collection Samples. OnLine Journal of Biological Sciences, (2022); 22(2): 237-246.


  • There are currently no refbacks.