Qualitative Phytochemical Analysis and Microbial Inhibitory Activities of Pacific Rain Tree (Samanea saman (Jacq.) Merr.) Pods

James Kennard Sanz Jacob, Eden S. David


Background: Crop diseases and human health are always at stake and the emerging problem on the use of synthetic anti-pathogens and medicine is one of the most difficult to combat. The first step towards determining such capabilities among plants is to determine their phytochemicals.

Methods: Eight preliminary phytochemical tests was done on Samanea saman which includes, test for alkaloids saponins, flavonoids, tannins, glycosides, steroids, terpenoids and resins. Powdered pods were subjected to ethanol and aqueous extraction. Extracts were also tested for its antifungal and anti-microbial properties against Fusarium oxysporumE. coli and S. aureus,respectively.

Results: Out of the eight phytochemical tests done, seven (7) were found to be present both on the ethanol and aqueous extracts namely, alkaloids, saponins, tannins, glycosides, steroids, terpenoids and resins. However, flavonoids is absent. The statistical results exhibited that there is a significant difference on the inhibitory effects against in-vitro bioassay of Fusarium oxysporumwhich is known to cause crop wilts and the two bacterial pathogens E. coli and S. aureus.

Conclusions: The presence of such phytochemicals in Samanea saman pods revealed that it can be a basis of new, natural and non-synthetic treatments. This finding suggests that its pods can be used as antibacterial and antifungal source.

Full Text:



Rongai D, Milano F, Sciò E. Inhibitory effect of plant extracts on conidial germination of the phytopathogenic fungus Fusarium oxysporum. American Journal of Plant Sciences, (2012); 3(12): 1693.

Hanaa RF, Abdou ZA, Salama DA, Ibrahim MA, Sror H. Effect of neem and willow aqueous extracts on Fusarium wilt disease in tomato seedlings: Induction of antioxidant defensive enzymes. Annals of Agricultural Sciences, (2011); 56(1): 1-7.s

Howell C. Mechanisms employed by Trichoderma species in the biological control of plant diseases: the history and evolution of current concepts. Plant Disease, (2003); 87(1): 4-10.

Obasi Nnamdi L, Egbuonu A, Ukoha P, Ejikeme P. Comparative phytochemical and antimicrobial screening of some solvent extracts of Samanea saman (fabaceae or mimosaceae) pods. African Journal of Pure and Applied Chemistry, (2010); 4206-212.

Durr P. The biology, ecology and agroforestry potential of the raintree, Samanea saman(Jacq.) Merr. Agroforestry Systems, (2001); 51(3): 223-237.

Zasloff M. Antimicrobial peptides of multicellular organisms. nature, (2002); 415(6870): 389-395.

Sofowora A. Recent trends in research into African medicinal plants. Journal of Ethnopharmacology, (1993); 38(2-3): 197-208.

Rios J, Recio M. Medicinal plants and antimicrobial activity. Journal of Ethnopharmacology, (2005); 100(1): 80-84.

Dwivedi S. Efficacy of some Medicinal Plant Extract Against Fusarium oxysporum f. sp. ciceri Causing Chickpea Wilt. Asian Journal of Crop Science, (2015); 7(2): 138.

Ghaima KK, Hashim NM, Ali SA. Antibacterial and antioxidant activities of ethyl acetate extract of nettle (Urtica dioica) and dandelion (Taraxacum officinale). Journal of Applied Pharmaceutical Science, (2013); 3(5): 96.

Yadav R, Agarwala M. Phytochemical analysis of some medicinal plants. Journal of Phytology, (2011); 3(12).

Fenwick DE, Oakenfull D. Saponin content of food plants and some prepared foods. Journal of the Science of Food and Agriculture, (1983); 34(2): 186-191.

Mann A, Banso A, Clifford L. An antifungal property of crude plant extracts from Anogeissus leiocarpus and Terminalia avicennioides. Tanzania Journal of Health Research, (2008); 10(1): 34-38.

Jacob JKS, David ES, Undan JR. Fungal inhibiting capacity of an ethnobotanical plant from Imugan, Nueva Vizcaya against Fusarium oxysporum and Fusarium moniliforme. International Journal of Agricultural Technology, (2016); 12(1): 167-171.

DOI: http://dx.doi.org/10.62940/als.v3i4.190


  • There are currently no refbacks.