Capability of Trichoderma viride to Produce Cellulolytic and Pectolytic Enzymes

Abdel Moneim Elhadi Sulieman, Siham M. Hakim, Wasima Alshammari, Nawaf I. Alshammari, Zakaria A. Salih


Background: Species of the genus Trichoderma have been used in the food and textile industries to produce cellulases and other enzymes that degrade complex polysaccharide structures. Trichoderma species have been utilized to make cellulases and other enzymes that break down intricate polysaccharide structures in the food and textile industries. The study investigated the nutritional requirements and the production of enzymes by the fungus Trichoderma viride fungus' ability to produce enzymes).

Methods: We used a medium supplemented with cellulolytic and pectic substances for enzyme production.

Results: The results of the study proved that. Methionine, Glutamic acid, and leucine effectively enhanced mycelial growth. Findings regarding impact of pH level on the development of the fungus T. viride indicated that the maximum growth was at pH 5.0. However, growth decreased dramatically with increasing pH values. We examined the power of the fungus T. viride to produce cellulolytic and pectolytic enzymes in various substrates in the current study.

Conclusion: The findings demonstrated that Pectin was the best substrate for pectolytic enzyme synthesis, whereas Carboxymethyl cellulose (CMC) was the best substrate for the cellulolytic enzyme.

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