Occurrence of HCV genotypes in different age groups of patients from Lahore, Pakistan
Abstract
Background: Hepatitis C virus is a small, enveloped single stranded, positive sense RNA virus. Different genotypes are distributed in different geographical areas of the world. Determination of HCV genotype is a powerful tool for the treatment of chronic and acute liver disease.
Method: The present study was carried out to find the occurrence of different HCV genotypes in the city of Lahore, a populous city of Pakistan from January 2010 to December 2010. Blood sample of patients positive for anti HCV by ELISA as well as HCV by PCR were collected and plasma was separated. HCV viral RNA load was analyzed in these samples using Real Time PCR. Qiagen HCV mini kit for RNA extraction and Qiagen HCV amplification kit for PCR amplification were used. Amplicons were subjected to HCV genotyping using Third Wave Technology.
Results: Among 489 patients, 211 (43.1%) patients were males and 278 (56.9%) were females. Occurrence of HCV in the age group of 36-45 years was 32.5 %. Occurrence of HCV genotype 1 was 9.6% (47), genotype 3a was 80.77% (395), genotype 3h was 1.0% (5) , genotype 4 was 4.9% (24), co-infection of genotypes 1 & 2 was 0.2% (01), co-infection of genotypes 1 & 3 was 0.6% (03) and co-infection genotypes 1 & 4 was 0.4% (02).
Conclusion: HCV genotype 3a is most prevalent HCV genotype in subjected population during said duration with most infected people from 26 to 35 years of age. Female population is having more of HCV infection as compared to males.
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Pavio N, Lai MM. The hepatitis C virus persistence: how to evade the immune system? Journal of biosciences, (2003); 28(3): 287-304.
Bhandari BN, Wright TL. Hepatitis C: an overview. Annual review of medicine, (1994); 46309-317.
Yap P, Kolberg J, Urdea M. Classification of hepatitis C virus into six major genotypes and a series of subtypes by phylogenetic analysis of the NS-5 region. Journal of General Virology, (1993); 742391-2399.
Simmonds P, Bukh J, Combet C, Deléage G, Enomoto N, et al. Consensus proposals for a unified system of nomenclature of hepatitis C virus genotypes. Hepatology, (2005); 42(4): 962-973.
Pawlotsky J-M. Mechanisms of antiviral treatment efficacy and failure in chronic hepatitis C. Antiviral research, (2003); 59(1): 1-11.
Saracco G, Ciancio A, Olivero A, Smedile A, Roffi L, et al. A randomized 4‐arm multicenter study of interferon alfa–2b plus ribavirin in the treatment of patients with chronic hepatitis C not responding to interferon alone. Hepatology, (2001); 34(1): 133-138.
McOmish F, Yap P, Dow B, Follett E, Seed C, et al. Geographical distribution of hepatitis C virus genotypes in blood donors: an international collaborative survey. Journal of Clinical Microbiology, (1994); 32(4): 884-892.
Chan S, McOmish F, Holmes E, Dow B, Peutherer J, et al. Analysis of a new hepatitis C virus type and its phylogenetic relationship to existing variants. The Journal of general virology, (1992); 731131-1141.
Silva CMDd, Costi C, Krug LP, Ramos AB, Grandi T, et al. High proportion of hepatitis C virus genotypes 1 and 3 in a large cohort of patients from Southern Brazil. Memórias do Instituto Oswaldo Cruz, (2007); 102(7): 867-870.
Bukh J, Purcell RH, Miller RH. At least 12 genotypes of hepatitis C virus predicted by sequence analysis of the putative E1 gene of isolates collected worldwide. Proceedings of the National Academy of Sciences, (1993); 90(17): 8234-8238.
Bonny C, Fontaine H, Poynard T, Hézode C, Larrey D, et al. Effectiveness of interferon plus ribavirin combination in the treatment of naive patients with hepatitis C virus type 5. A French multicentre retrospective study. Alimentary pharmacology & therapeutics, (2006); 24(4): 593-600.
Pybus OG, Markov PV, Wu A, Tatem AJ. Investigating the endemic transmission of the hepatitis C virus. International journal for parasitology, (2007); 37(8): 839-849.
Sultanat C, Oprisan G, Szmal C, Vagu C, Temereanca A, et al. Molecular epidemiology of hepatitis C virus strains from Romania. Journal of Gastrointestinal & Liver Diseases, (2011); 20(3).
Ali A, Ahmed H, Idrees M. Molecular epidemiology of Hepatitis C virus genotypes in Khyber Pakhtoonkhaw of Pakistan. Virology Journal, (2010); 7(1): 203.
Tokita H, Okamoto H, Tsuda F, Song P, Nakata S, et al. Hepatitis C virus variants from Vietnam are classifiable into the seventh, eighth, and ninth major genetic groups. Proceedings of the National Academy of Sciences, (1994); 91(23): 11022-11026.
de Lamballerie X, Charrel RN, Attoui H, De Micco P. Classification of hepatitis C virus variants in six major types based on analysis of the envelope 1 and nonstructural 5B genome regions and complete polyprotein sequences. Journal of General Virology, (1997); 78(1): 45-51.
Abdulkarim AS, Zein NN, Germer JJ, Kolbert CP, Kabbani L, et al. Hepatitis C virus genotypes and hepatitis G virus in hemodialysis patients from Syria: identification of two novel hepatitis C virus subtypes. The American journal of tropical medicine and hygiene, (1998); 59(4): 571-576.
Idrees M. Detection of Six Serotypes of HCV in anti-HCV Positive Patients and rate of ALT/AST abnormalities. Pak J Microbiol, (2001); 261-65.
Idrees M, Riazuddin S. Frequency distribution of hepatitis C virus genotypes in different geographical regions of Pakistan and their possible routes of transmission. BMC Infectious Diseases, (2008); 8(1): 69.
Poynard T, Bedossa P, Chevallier M, Mathurin P, Lemonnier C, et al. A comparison of three interferon alfa-2b regimens for the long-term treatment of chronic non-A, non-B hepatitis. New England Journal of Medicine, (1995); 332(22): 1457-1463.
McHutchison J, Poynard T, Davis G, Esteban-Mur R, Harvey J, et al. Evaluation of hepatic HCV RNA before an after treatment with interferon alfa 2B or combined with ribavirin in chronic hepatitis; 1999. WB SAUNDERS CO INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 USA. pp. 363A-363A.
Dusheiko G, Main J, Thomas H, Reichard O, Lee C, et al. Ribavirin treatment for patients with chronic hepatitis C: results of a placebo-controlled study. Journal of hepatology, (1996); 25(5): 591-598.
Nousbaum J-B, Pol S, Nalpas B, Landais P, Berthelot P, et al. Hepatitis C virus type 1b (II) infection in France and Italy. Annals of internal medicine, (1995); 122(3): 161-168.
Ahmad W, Ijaz B, Javed FT, Jahan S, Shahid I, et al. HCV genotype distribution and possible transmission risks in Lahore, Pakistan. World journal of gastroenterology: WJG, (2010); 16(34): 4321.
Ali M, Kanwal L, Tassaduqe K, Iqbal R. Prevalence of Hepatitis C Virus (HCV) in Relation to Its Promotive Factors Among Human Urban Population of Multan, Pakistan. European Journal of General Medicine, (2009); 6(2).
Idrees M, Riazuddin S. A study of best positive predictors for sustained virologic response to interferon alpha plus ribavirin therapy in naive chronic hepatitis C patients. BMC gastroenterology, (2009); 9(1): 5.
Butt S, Idrees M, Akbar H, Awan Z, Afzal S, et al. The changing epidemiology pattern and frequency distribution of hepatitis C virus in Pakistan. Infection, Genetics and Evolution, (2010); 10(5): 595-600.
Campiotto S, Pinho J, Carrilho F, Da Silva L, Souto F, et al. Geographic distribution of hepatitis C virus genotypes in Brazil. Brazilian Journal of Medical and Biological Research, (2005); 38(1): 41-49.
DOI: http://dx.doi.org/10.62940/als.v1i2.22
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