Inoculation of wheat with Azospirillum sp. bacteria and study of their germinated portions effects on common carp performance and health aspects

Shahen Kamil Talabani, Shadia Ali Abid, Nasreen MohiAlddin Abdulrahman, Hawar Sleman Hama Halshoy, Shram Hoshyar Karim


Background: As the germinated wheat regarded as a natural source of prebiotic, and the importance of prebiotic on performance and health of fish, so the effect of feed containing various amount of germinated wheat as a natural source of prebiotic, on growth performance, blood chemical, immunity indices, health and biological parameters in common carp (Cyprinus carpio L.).

Methods: The first experiment started by planting pots in the plastic house, the pots arranged according to a Randomized Complete Design (RCD) layout with three replications. In each replication, ten seeds planted in the pots. The replications divided and arranged on 2 treatments, a control treatment taken in which seeds will be not inoculated with Azospirillum bacteria, the second treatment inoculated seeds with Azospirillum species which isolated from soil and culturing on its selective media then incubated at 28 0C for 7 days, after that the colonies of bacteria activated in its broth culture media at 28 0C for 3 days and it will be used as a liquid inoculum for the seeds. The second experiment the rearing of fish conducted at the Fish diseases Laboratory, College of Veterinary Medicine/ University of Sulaimani. 100 common carp (Cyprinus. carpio L.) reared for70 days.

Result: Negative control T1 was higher significantly in Hepatic somatic index, T1 and T3 in Gills somatic index. T4 increased significantly in FCR, and Intestine Length index. T3 and T5 increased growth performance parameters and Feed and Protein efficiency ratio. T1, T3, and T4 increased the Intestine weight index. T4 and T5 increased the RBC. T3, T4, and T5 increased the ALT, and Globulin. MCH, MCHC, Lymphocytes, and CKI increased significantly in T5. All treatment groups increased each of Condition factor, and Granulocytes as compared to negative control. No significant differences seen in both Kidney, Spleen somatic index, Hb.

Conclusion: According to the results obtained the inoculation of wheat with Azospirillum sp. bacteria enhance the common carp performance and health aspects by means of some biological parameters.

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FAO. (2022). The State of World Fisheries and Aquaculture 2022. Towards Blue Transformation. Rome, FAO. 266 pp.

Abdulrahman NM. Effect of germinated barely and earth apple (Helianthustuberosus) powders in some physio-biological indices of common carp (Cyprinus carpio L.). Iranian Journal of Veterinary Medicine, (2022);16(2): 119-125.

Mohammad MA. Effect of using germinated for common vetch seeds Vicia sativa on growth performance of common carp Cyprinus carpio L. Iraqi Journal of Veterinary Medicine, (2017);41(1):118-124.

Vo BV, Siddik MAB, Chaklader MR, Fotedar R, Nahar A, Foysal MJ. Growth and health of juvenile barramundi (Lates calcarifer) challenged with DO hypoxia after feeding various inclusions of germinated, fermented and untreated peanut meals. PLoS ONE, (2020);15(4): e0232278.

Suwara I, Lenart S, Gawrońska-Kulesza A. Growth and yield of winter wheat after 50 years of different fertilization and crop rotation. Acta Agrophysica, (2007);153: 695-704.

Soliman MAE. Wheat Response to Azospirillum Inoculation, Organic Amendments and Mineral Nitrogen Fertilizer with Application of 15N Stable Isotope Technique. Journal of Soil Sciences and Agricultural Engineering, Mansoura University, (2018); 9 (12): 821 - 827.

Bielińska EJ,Mocek-Płóciniak A. Impact of the tillage system on the soil enzymatic activity. Archive Environmental Protection, (2012); 38(1): 75-82.

Naiman AD, Latrónico A,Garcıa de Salamone IE. Inoculation of wheat with Azospirillum brasilense and Pseudomonas fluorescens: Impact on the production and culturable rhizosphere microflora. European Journal of Soil Biology, (2009); 45: 44-51.

Cardarelli M, Woo SL, Rouphael Y, Colla G. Seed Treatments with Microorganisms Can Have a Biostimulant Effect by Influencing Germination and Seedling Growth of Crops. Plants, (2022); 11: 259.

Abdulrahman NM, Sadeeq ET. The Effects of Using Germinated Wheat on Cyprinuscarpio L. Productive and Developmental Performances. ProEnvironment, (2022); 15(50): 162 - 169

Uten F. Standard methods and terminology in finfish nutrition Proc World Symp. Finfish Nutrition and Fish– Technology, 1978: 20-23.

Hassan BR, Abdulrahman NM, Salman NA. Physiological impacts of using clove powder and oil as fish anesthetic on young common carp (Cyprinus carpio L.). Basrah Journal of Veterinary Research, Proceeding of 5th International scientific Conference, College of Veterinary Medicine University of Basrah, Iraq. (2016); 15(3): 293-311.

Lagler KF. (1956). Fresh water Fishery Biology. W.C. Brown Company, Dubuque, IA., USA. 131-135: 159-166.

El-katatny MH, Idres MM. Effects of single and combined inoculations with Azospirillumbrasilense and Trichoderma harzianum on seedling growth or yield parameters of wheat (Triticum vulgaris L., Giza 168) and corn (Zea mays L., hybrid 310). Journal of Plant Nutrition, (2014);37(12): 1913-1936(24).

Mehanni MM, El-Katatny H. Evaluation of the antifungal activity of some Azospirillum strains for their possible role as biocontrol agents. (2023).

Gaspareto RN, Jalal A, Ito, WCN, Oliveira CEdS, Garcia CMdP, Boleta EHM, Rosa PAL, Galindo FS, Buzetti S, Ghaley BB. Inoculation with Plant Growth-Promoting Bacteria and Nitrogen Doses Improves Wheat Productivity and Nitrogen Use Efficiency. Microorganisms, (2023); 11: 1046.

Abdulrahman NM, Sleman HD, Ramzi DOM, Hama-Salih HA. Effect of Chlorella microalgae and germinated barley powder on performance, some health indices, and meat hygiene parameters of common carp (Cyprinus carpio). Basrah Journal of Veterinary Research, Proceeding of the 17th International Conference. College of Veterinary Medicine. University of Basrah. Iraq. (2020); 19(3): 218-213.

Al-Amili HA. (2017). Effect of different levels of earth apple and germinated barley on growth performance and some blood picture measurement of common carp Cyprinus carpio L. PhD dissertation, college of Agriculture at University of Anbar. 120 pp.

Assenova B, Okuskhanova E, Rebezov M, Zinina O, Baryshnikova N, Vaiscrobova E, Kasatkina E, Shariati MA, Khan MU,Ntsefong GN. Effect of germinated wheat (Triticumaestivum) on chemical, amino acid and organoleptic properties of meat pate. Potravinarstvo Slovak Journal of Food Sciences, (2020); 14: 580-586.

Boleta EHM, Shintate GF, Jalal A, Santini, JMK, Rodrigues WL, Lima BH, Arf O, Silva MR, Buzetti S, Teixeira FMC. Inoculation With Growth-Promoting Bacteria Azospirillum brasilense and Its Effects on Productivity and Nutritional Accumulation of Wheat Cultivars. Front. Sustain. Food Systems, (2020); 4: 607262.

Rico D, Peñas E, García MDC, Martínez-Villaluenga C, Rai DK, Birsan RI, Martín-Diana AB. Sprouted Barley Flour as a Nutritious and Functional Ingredient. Foods, (2020); 9(3): 296.

Iraporda C, Rubel IA, Manrique GD, Abraham AG. Influence of inulin rich carbohydrates from Jerusalem artichoke (Helianthus tuberosus L.) tubers on probiotic properties of Lactobacillus strains. LWT - Food Science and Technology, (2019); 101: 738-746.

Abedalhammed HS, Hassan SM, Naser AS, AL-Maathedy MH, Abdulateef SM, Mohammed TT. Effect of using Jerusalem artichoke (Helianthus tuberosus L.) and Sprout germinated barley as a prebiotics to the diets of common carp (Cyprinus carpio L.) fingerling on the rates of apparent digestible coefficient and apparent protein digestible. Eurasia Journal of Bioscience, (2020); 14: 3741-3745.

Dawood MA, Metwally AES, El-Sharawy ME, Atta AM, Elbialy ZI, Abdel-Latif HM, Paray BA. The role of β-glucan in the growth, intestinal morphometry, and immune-related gene and heat shock protein expressions of Nile tilapia (Oreochromis niloticus) under different stocking densities. Aquaculture, (2020); 523: 735205.


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