N-cadherin protein-immuno-histochemical expression of the blood-testis barrier in male mice fed with diet minced with insecticide Bifenthrin
Abstract
Background: Testis play role as endocrine organ by regulate and synthesis of hormones, and genital role as germ sells production (spermatogenesis) for reproduction process. The Sertoli cells in the seminiferous tubule with basal lamina and endothelium of vesicles, form a special structure called blood-testis-barrier (BTB) withe protection function and specific properties that similar to blood tissue barriers of other body organs.
Methods: Forty male healthy Swiss albino mice weighing 24-30 g will be kept in 2 groups (20 for each) in the labs of Al-Nahrain University College of medicine for 6 weeks from 1 December 2022_15 Jan 2023. Regular rodent food and water will be provided and Grouping as: Group A food minced with concentration of 400mg/kg of Bifenthrin insecticides and Group B have normal food.
Results: The histological study showed partial depletion testis cells in all mice testes with non-significant decrease in interstitial space that has Leydig cells between seminiferous tubule (ST) in testis of male mice that were fed with Bifenthrin diet. There were less cadherin expression in the BTB indicating that the Bifenthrin has stressful stimulus to potentiate apoptosis in the genital and somatic (Sertoli) cells of the testis that eventually impact the cadherin protein. The Bifenthrin has as stimulant chemical factor for more apoptosis in the ST cells death with subsequently testicular atrophy and subsequently infertility. The Bifenthrin treated male mice shows significant diminish positive values of N-cadherin expression that appear as less brown coloration of immunohistochemical biomarker expression of N-cadherin in the ST cells of the testis that was best evaluated by applying Aperio software program.
Conclusions: The N-cadherin protein has pivotal role in regulation the adhesiveness of the germinative-epithelial cells in ST of the testis. The Bifenthrin insecticides as an endocrine disrupter material has great effects on spermatogenesis and tissue damage.
Full Text:
PDFReferences
Stanton P. Regulation of the blood-testis barrier. In Seminars in cell & developmental biology. Academic Press, (2016); 59: 166-173.
Mruk D, Cheng CY. The mammalian blood-testis barrier: its biology and regulation. Endocrine reviews, (2015); 36(5): 564-591.
Griswold M. The central role of Sertoli cells in spermatogenesis. In Seminars in cell & developmental biology. Academic Press, (1998); 9(4): 411-416.
Moreira S, Pereira S, Seco-Rovira V, Oliveira P, Alves M, Pereira M. Pesticides and male fertility: A dangerous crosstalk. Metabolites, (2021); 11(12): 799.
Lie P, Cheng C, Mruk, D. The biology of the desmosome-like junction: a versatile anchoring junction and signal transducer in the seminiferous epithelium. International review of cell and molecular biology, (2011); 286: 223-269.
Aydin S, Billur D, Kizil S, Ozkavukcu S, Topal F, Aydos K, Erdemli E. Evaluation of blood–testis barrier integrity in terms of adhesion molecules in nonobstructive azoospermia. Andrologia, (2020); 52(7): e13636.
Mruk D, Cheng C. The mammalian blood-testis barrier: its biology and regulation. Endocrine reviews, (2015); 36(5): 564-591.
Hess R, De Franca L. Spermatogenesis and cycle of the seminiferous epithelium. Molecular mechanisms in spermatogenesis, (2009); 1-15.
Smith L, Walker W. The regulation of spermatogenesis by androgens. In Seminars in cell & developmental biology. Academic Press, (2014); 30: 2-13.
Wang R, Yeh S, Tzeng C, Chang C. Androgen receptor roles in spermatogenesis and fertility: lessons from testicular cell-specific androgen receptor knockout mice. Endocrine reviews, (2009); 30(2): 119-132.
Wheelock M , Shintani Y, Maeda M, Fukumoto Y, Johnson K. Cadherin switching. Journal of cell science, (2008); 121(6): 727-735.
Park H, Kim J, Lee R, Song H. Cisplatin induces apoptosis in mouse neonatal testes organ culture. International Journal of Molecular Sciences, (2022); 23(21): 13360.
O'Donnell L, Stanton P, de Kretser D. Endocrinology of the male reproductive system and spermatogenesis, (2015); 6(6): 45-67.
Moreira S, Pereira S, Seco-Rovira, V, Oliveira P, Alves M, Pereira M. Pesticides and male fertility: A dangerous crosstalk. Metabolites, (2021); 11(12): 799.
Yawer A, Sychrová E, Labohá P, Raška J, Jambor T, Babica P, Sovadinová I. Endocrine-disrupting chemicals rapidly affect intercellular signaling in Leydig cells. Toxicology and Applied Pharmacology, (2020); 404: 115177.
Rato L, Alves M, Socorro S, Duarte A, Cavaco J, Oliveira P. Metabolic regulation is important for spermatogenesis. Nature Reviews Urology, (2012); 9(6): 330-338.
Cardoso A, Alves M, Mathur P, Oliveira P, Cavaco J, Rato L. Obesogens and male fertility. Obesity Reviews, (2017); 18(1): 109-125.
Babakhanzadeh E, Nazari M, Ghasemifar S, Khodadadian A. Some of the factors involved in male infertility: a prospective review. International journal of general medicine, (2020); 29-41.
Urióstegui-Acosta M, Tello-Mora P, de Jesús Solís-Heredia M, Ortega-Olvera J M, Piña-Guzmán B, Martín-Tapia D, Quintanilla-Vega B. Methyl parathion causes genetic damage in sperm and disrupts the permeability of the blood-testis barrier by an oxidant mechanism in mice. Toxicology, (2020); 438: 152463.
Ham J, You S, Lim W, Song G. Bifenthrin impairs the functions of Leydig and Sertoli cells in mice via mitochondrion-endoplasmic reticulum dysregulation. Environmental Pollution, (2020); 266: 115174.
Wang Q, Wang H, Shen J, Zhan R, Hong J, Li Z, Xu L. The anti-androgenic effects of cypermethrin mediated by non-classical testosterone pathway activation of mitogen-activated protein kinase
cascade in mouse Sertoli cells. Ecotoxicology and Environmental Safety, (2019); 177: 58-65.
Erthal R, Staurengo-Ferrari V, Luiz K, Cunha F, Pescim R, Fernandes G. A. Exposure to low doses of malathion during juvenile and peripubertal periods impairs testicular and sperm parameters in rats: role of oxidative stress and testosterone. Reproductive Toxicology, (2020); 96: 17-26.
Ali W, Ma Y, Zhu J, Zou H, Liu Z. Mechanisms of cadmium-induced testicular injury: A risk to male fertility. Cells, (2022); 11(22): 3601.
Hashim Z, Kareem H, Jaafar H. The Evaluation of Placental Vascular Generation and Placental Apoptosis in Preterm and Post-date Placentae in Relation to Apgar Score at Birth. Medical Journal of Babylon, (2016); 13(1): 79-84.
Taeeh S, Jaafar H T, Jaafar H A, Jarullah H A. Effects of Dark-Light Cycle on the Histological and Immunohistochemical P73 Marker Expression of Cajal Cells in the Cerebral Cortex of Mice. HIV Nursing, (2023); 23(3): 2098-2106.
Wei C, Wang X, Yao X, Xi F, He Y, Xu Y, Yu T. Bifenthrin induces fat deposition by improving fatty acid uptake and inhibiting lipolysis in mice. Journal of agricultural and food chemistry, (2019); 67(51): 14048-14055.
Ong-Artborirak P, Boonchieng,W. Juntarawijit,Y, Juntarawijit C. Potential effects on mental health status associated with occupational exposure to pesticides among Thai farmers. International Journal of Environmental Research and Public Health, (2022); 19(15): 9654.
Khan N, Kennedy A, Cotton J, Brumby S. A pest to mental health? Exploring the link between exposure to agrichemicals in farmers and mental health. International journal of environmental research and public health, (2019); 16(8): 1327.
Titi-Lartey O, Khan Y. Embryology, testicle, (2020); 5(8): 44-89.
Hadi U, Jaafar H. The Effect Of Light/Dark Cycle On Blood Follicular Barrier Laminin Expression In Ovary Of The Mice. Biochemical & Cellular Archives, (2019); 19(2).
Mruk D, Cheng C. Sertoli-Sertoli and Sertoli-germ cell interactions and their significance in germ cell movement in the seminiferous epithelium during spermatogenesis. Endocrine reviews, (2004); 25(5): 747-806.
Mruk D, Cheng C. The mammalian blood-testis barrier: its biology and regulation. Endocrine reviews, (2015); 36(5): 564-591.
DOI: http://dx.doi.org/10.62940/als.v10i4.2338
Refbacks
- There are currently no refbacks.