Evaluating the Antioxidant Capacity, Phenolic Contents, and Anticancer Potential of Caralluma europaea Extracts

Omar M. Atrooz, Makarem Al-Awaida, Wajdy Al-Awaida, Hamzeh J. Al-Ameer, Hamdi Uysal

Abstract


Background: Caralluma europaea(C. europaea) is a genus of shrubs in the Apocynaceae family, endemic to the Mediterranean region. Assessing the anticancer properties, total phenolic and flavonoid content, and antioxidant activity of differentC. europaeaextracts were the aim of this study.

Methods: To determine antioxidant activity, we used three free radical scavenging assays: DPPH (2,2-Diphenyl-1-picrylhydrazyl), hydrogen peroxide (H2O2), and nitric oxide (NO) assays. Total phenolic and flavonoid contents were measured using colorimetric methods. The anti-important activity was evaluated by MTT assay.

Results: Our findings showed that the efficacy of the extract in MCF7 cells decreased in the system containing chloroform, methanol, ethyl acetate, and water. The strength of the extract for U87 cells decreased according to methanolic, chloroform and water, where ethyl acetate was the strongest. The highest total phenolic and flavonoid content was found in methanol extract. All extracts showed a high scavenging activity of DPPH. Methanol extract showed the highest antioxidant activity in the NO assay of the solution, followed by ethyl acetate, chloroform, and aqueous extract. In the H2O2-based antioxidant activity assay, the methanol extract again showed the highest activity, followed by ethyl acetate, chloroform, and aqueous extracts.

Conclusions: Extracts ofC. europaeahave shown great potential as natural antioxidants and anticancer agents in this study, suggesting that they deserve further investigation into the food and pharmaceutical fields.

Keywords: Phenolic content; Flavonoid content; Antioxidant activity; Anti-cancer activity

Editorial Note on Version of Record
03 Oct 2026: This article has been corrected. See https://doi.org/10.62940/als.v13i0.4377 for more information.


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References


Willcox ML, Elugbaju C, Al-Anbaki M, Lown M, Graz B. Effectiveness of medicinal plants for glycaemic control in type 2 diabetes: an overview of meta-analyses of clinical trials. Frontiers in Pharmacology, (2021); 12:777561.

Alqahtani AS, Ullah R, Shahat AA. Bioactive constituents and toxicological evaluation of selected antidiabetic medicinal plants of Saudi Arabia. Evidence-Based Complementary and Alternative Medicine, (2022); 2022(1): 7123521

Hussein RA, El-Anssary AA. Plants secondary metabolites: the key drivers of the pharmacological actions of medicinal plants. Herbal medicine, (2019); 1(3): 11-30.

Bruce SO, Onyegbule FA. (2021) Biosynthesis of natural products. In: Bioactive CompoundsBiosynthesis, Characterization and Applications. IntechOpen.

Aryal S, Baniya MK, Danekhu K, Kunwar P, Gurung R, et al. Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from Western Nepal. Plants, (2019); 8(4): 96.

Peeters K, Miklav?i? VA, Esakkimuthu ES, Schwarzkopf M, Tavzes ?. The Valorisation of Olive Mill Wastewater from Slovenian Istria by Fe3O4 Particles to Recover Polyphenolic Compounds for the Chemical Specialties Sector. Molecules, (2021); 26(22): 6946.

Sahiner M, Yilmaz AS, Gungor B, Ayoubi Y, Sahiner N. Therapeutic and nutraceutical effects of polyphenolics from natural sources. Molecules, (2022); 27(19): 6225.

Souri E, Amin G, Farsam H, Jalalizadeh H, Barezi S. Screening of thirteen medicinal plant extracts for antioxidant activity. Iranian Journal of Pharmaceutical Research, (2008); 7(2): 149-154.

Spiegel M. Current trends in computational quantum chemistry studies on antioxidant radical scavenging activity. Journal of Chemical Information and Modeling, (2022); 62(11): 2639-2658.

Atrooz OM, Al-Maitah SZ. Characterization of the crude extract of Portulaca oleracea and the determination of the polyphenol oxidase kinetics in the presence of Cu and Zn. Journal of Applied Biology and Biotechnology, (2022); 10(3): 28-33.

Lanchhana DS, Ranjit M, Kumar MS. Anticancer activity study of some selected Indian medicinal plants used traditionally. Journal of Pharmaceutical Negative Results, (2023); 14(2): 1123-1132.

Deep A, Kumar D, Bansal N, Narasimhan B, Marwaha RK, et al. Understanding mechanistic aspects and therapeutic potential of natural substances as anticancer agents. Phytomedicine Plus, (2023); 3(2): 100418.

Kowalczyk T, Merecz-Sadowska A, Rijo P, Mori M, Hatziantoniou S, et al. Hidden in plantsA review of the anticancer potential of the Solanaceae family in in vitro and in vivo studies. Cancers, (2022); 14(6): 1455.

Wani AK, Akhtar N, Mir TU, Singh R, Jha PK, et al. Targeting apoptotic pathway of cancer cells with phytochemicals and plant-based nanomaterials. Biomolecules, (2023); 13(2): 194.

Bushnak R, El Hajj M, Jaber A. A review on the antidiabetic potential of medicinal plants. Journal of Medicine and Health, (2021); 4: 172-189.

Ouassou H, Bouhrim M, Kharchoufa L, Imtara H, Daoudi N, et al. Caralluma europaea (Guss) N.E. Br.: A review on ethnomedicinal uses, phytochemistry, pharmacological activities, and toxicology. Journal of Ethnopharmacology, (2021); 273: 113769.

Dra LA, Aghraz A, Boualy B, Oubaassine S, Barakate M, et al. Chemical characterization and in vitro antimicrobial activity of Caralluma europaea essential oil and its synergistic potential with conventional antibiotics. Journal of Advances in Medical and Pharmaceutical Sciences, (2018); 19(4): 1-11.

Bourhia M, Slighoua M, Ibnemoussa S, Bari A, Ullah R, et al. Phytochemical study on antioxidant and antiproliferative activities of Moroccan Caralluma europaea extract and its bioactive compound classes. Evidence-Based Complementary and Alternative Medicine, (2020); 2020(1): 2020.

Amrati FE, Chebaibi M, Galvao de AR, Conte R, Slighoua M, et al. Phenolic composition, wound healing, antinociceptive, and anticancer effects of Caralluma europaea extracts. Molecules, (2023); 28(4):1780.

Amrati FE-Z, Bourhia M, Saghrouchni H, Slighoua M, Grafov A, et al. Caralluma europaea (Guss.) N.E.Br.: anti-inflammatory, antifungal, and antibacterial activities against nosocomial antibiotic-resistant microbes of chemically characterized fractions. Molecules, (2021); 26(3):636.

Baliyan S, Mukherjee R, Priyadarshini A, Vibhuti A, Gupta A, et al. Determination of antioxidants by DPPH radical scavenging activity and quantitative phytochemical analysis of Ficus religiosa. Molecules, (2022); 27(4): 1326.

Sreejayan XX, Rao MN. Nitric oxide scavenging by curcuminoids. Journal of pharmacy and Pharmacology, (1997); 49(1): 105-107.

Al-Awaida W, Al-Hourani BJ, Akash M, Talib WH, Zein S, et al. In vitro anticancer, anti-inflammatory, and antioxidant potentials of Ephedra aphylla. Journal of cancer research and therapeutics, (2018); 14(6): 1350-1354.

Mohan SC, Balamurugan V, Salini ST, Rekha R. Metal ion chelating activity and hydrogen peroxide scavenging activity of medicinal plant Kalanchoe pinnata. Journal of Chemical and Pharmaceutical Research, (2012); 4(1): 197-202.

Alfarrayeh I, Tarawneh K, Almajali D, Al-Awaida W. Evaluation of the Antibacterial and Antioxidant properties of the Methanolic extracts of four Medicinal plants selected from Wadi Al-Karak, Jordan related to their Phenolic contents. Research Journal of Pharmacy and Technology, (2022); 15(5): 2110-2116.

Samatha T, Shyamsundarachary R, Srinivas P, Swamy NR. Quantification of total phenolic and total flavonoid contents in extracts of Oroxylum indicum L. Kurz. Asian Journal of Pharmaceutical and Clinical Research, (2012); 5(4): 177-179.

Sireesha M, Nadh RV, Babu K, Sreenivasulu M. Phytochemical library of caralluma genus. International Journal of Research in Pharmaceutical Sciences, (2018); 9(4): 1201-1213.

Malladi S, Ratnakaram VN, Suresh BK, Pullaiah T. Phytochemical screening of Caralluma lasiantha: Isolation of C21 pregnane steroid. Oriental Journal of Chemistry, (2017); 33(2): 963.

De Luca I, Di Cristo F, Valentino A, Peluso G, Di Salle A, et al. Food-derived bioactive molecules from mediterranean diet: nanotechnological approaches and waste valorization as strategies to improve human wellness. Polymers, (2022); 14(9): 1726.

Zazouli S, Chigr M, Jouaiti A. Effect of polar and nonpolar solvent on total phenolic and antioxidant activity of roots extracts of Caralluma europaea. Der Pharma Chemica, (2016); 8(11): 191-196.

Hassanpour SH, Doroudi A. Review of the antioxidant potential of flavonoids as a subgroup of polyphenols and partial substitute for synthetic antioxidants. Avicenna Journal of Phytomedicine, (2023); 13(4): 354.

Al-Nemi R, Makki AA, Sawalha K, Hajjar D, Jaberko M. Untargeted metabolomic profiling and antioxidant capacities of different solvent crude extracts of Ephedra foeminea. Metabolites, (2022); 12(5): 451.




DOI: https://doi.org/10.62940/als.v11i4.3296

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