Effects of Sapodilla Leaf Extract (Manilkara zapota L.) Administration on the Histopathological Presentation of Kidney Tubules and Glomeruli in Alloxan-Induced Mice
Abstract
Background: Diabetes mellitus is a metabolic disorder characterized by high blood glucose levels or insulin deficiency. Sapodilla (Manilkara zapota L.) leaves are known to be potentially utilized as an alternative remedy for diabetes. The objective of this research is to investigate the effect of Sapodilla leaf extract on the improvement of histopathological presentation of kidneys in alloxan-induced diabetic mice.
Methods: This research used 3 months old male mice weighing 25-35g. A total of 25 mice were used, with 5 mice per each group. The treatments employed included P1 (Sapodilla leaf extract at a dosage of 100 mg/kg BW), P2 (Sapodilla leaf extract at a dosage of 300 mg/kg BW), K+ (Pioglitazone at a dosage of 2 mg/kg BW), K0 (No treatment administered), and K- (Diabetic control). Kidney sample collection was conducted on the 14th day after therapy administration. Histopathological samples were examined using a trinocular microscope. Data analysis was conducted using the Kruskal-Wallis method, followed by the Mann-Whitney test.
Results: The results of the Kruskal-Wallis test with the parameter of necrosis indicate a p-value of .001. The degeneration of cells shows a p-value of .001, while congestion shows a p-value of .001 as well. The Mann-Whitney test was conducted on the parameters of necrosis, cell degeneration, and congestion. The results indicated that the P1 treatment group was not significantly different from the P2 and K+ groups for cell degeneration and congestion; however, a significant difference was observed between P1 and K+ for the necrosis parameter.
Conclusion: The study concludes the data analysis revealed that the administration of sapodilla leaf extract can ameliorate kidney damage in diabetic mice induced with alloxan.
Keywords: Alloxan; Diabetes; Kidney Histopathology; Sapodilla leaf extract
Full Text:
PDFReferences
Banday MZ, Sameer AS, Nissar S. Pathophysiology of diabetes: An overview. Avicenna Journal of Medicine, (2020); 10(4): 174-188.
Singh AK, Yadav D, Sharma N, Jin JO. Dipeptidyl peptidase (DPP)-IV inhibitors with antioxidant potential isolated from natural sources: A novel approach for the management of diabetes. Pharmaceuticals, (2021); 14(6): 586.
Balaji R, Duraisamy R, Kumar MP. Complications of diabetes mellitus: A review. Drug Invention Today, (2019); 12(1): 98-103.
Unsal V, Cicek M, Sabancilar I. Toxicity of carbon tetrachloride, free radicals and role of antioxidants. Reviews on Environmental Health, (2021); 36(2): 279-295.
Deprem T, Aksu SI, Taşçi SK, Bingöl SA, Gülmez N, Aslan Ş. Immunohistochemical distributions of HGF and PCNA in the kidneys of diabetic and non-diabetic mice. Kafkas Universitesi Veteriner Fakultesi Dergisi. (2020); 26(3): 321-327.
Lodhi AH, Ahmad FUD, Furwa K, Madni A. Role of oxidative stress and reduced endogenous hydrogen sulfide in diabetic nephropathy. Drug Design, Development and Therapy, (2021); 15: 1031-1043.
Noor T, Hanif F, Kiran Z, Rehman R, Khan MT, et al. Relation of copeptin with diabetic and renal function markers among patients with diabetes mellitus progressing towards diabetic nephropathy. Archives of Medical Research, 2020; 51(6): 548-555.
Selby NM, Taal MW. An updated overview of diabetic nephropathy: Diagnosis, prognosis, treatment goals and latest guidelines. Diabetes, Obesity and Metabolism, (2020); 22: 3-15.
Kim H, Bae YU, Jeon JS, Noh H, Park HK, et al. The circulating exosomal microRNAs related to albuminuria in patients with diabetic nephropathy. Journal of Translational Medicine, (2019); 17: 1-11.
Chen HY, Pan HC, Chen YC, Chen YC, Lin YH, et al. Traditional Chinese medicine use is associated with lower end-stage renal disease and mortality rates among patients with diabetic nephropathy: a population-based cohort study. BMC Complementary and Alternative Medicine, (2019); 19(1): 1-13.
Cheng S, Hou G, Liu Z, Lu Y, Liang S, et al. Risk prediction of in-hospital mortality among patients with type 2 diabetes mellitus and concomitant community-acquired pneumonia. Annals of Palliative Medicine, (2020); 9(5): 3313-3325.
Tynjälä A, Forsblom C, Harjutsalo V, Groop PH, Gordin D. Arterial stiffness predicts mortality in individuals with type 1 diabetes. Diabetes Care, (2020); 43(9): 2266-2271.
Merrill AE, Chambliss AB. Water and electrolyte balance. In Contemporary Practice in Clinical Chemistry. In: Academic Press, (2020): 651-663.
Wulczyn KE, Zhao SH, Rhee EP, Kalim S, Shafi T. Trajectories of uremic symptom severity and kidney function in patients with chronic kidney disease. Clinical Journal of the American Society of Nephrology, (2022); 17(4): 496-506.
Solikhah TI, Setiawan B, Ismukada DR. Antidiabetic activity of papaya leaf extract (Carica Papaya L.) isolated with maceration method in alloxan-induces diabetic mice. Systematic Reviews in Pharmacy, (2020); 11(9): 774-778.
Ahmed YM, Orfali R, Abdelwahab NS, Hassan HM, Rateb ME, AboulMagd AM. Partial synthetic pparƳ derivative ameliorates aorta injury in experimental diabetic rats mediated by activation of miR-126-5p Pi3k/AKT/PDK 1/mTOR expression. Pharmaceuticals, (2022); 15(10): 1-19.
Wang L, Wang J, Lin C, Wang F, Li X, et al. Simultaneous quantification of pioglitazone and omarigliptin in rat plasma by UHPLC-MS/MS and its application to pharmacokinetic study after coadministration of the two drugs. Journal of Analytical Methods in Chemistry, (2021); 2021: 1-9.
Khairullah AR, Solikhah TI, Ansori ANM, Hanisia RH, Puspitarani GA, et al. Medicinal importance of Kaempferia galanga L. (Zingiberaceae): A comprehensive review. Journal of Herbmed Pharmacology, (2021); 10(3): 281-288.
Ansori ANM, Kharisma VD, Solikhah TI. Medicinal properties of Muntingia calabura L. Research Journal of Pharmacy and Technology, (2021); 14(8): 4505-4508.
Rafif KA, Intan ST, Muhammad AAN, Mulyo RH. A review on phytochemistry and pharmacology of Eclipta alba L. A valuable medicinal plant. Research Journal of Biotechnology, (2022); 17(3): 134-139.
Solikhah TI, Wijaya TA, Pavita DA, Kusnandar R, Wijaya A, et al. The effect of sapodilla leaf extract (Manilkara zapota L.) on lipid profiles of alloxan-induced diabetic mice. Pharmacognosy Journal, (2023); 15(2): 286-289.
Khairullah AR, Solikhah TI, Ansori ANM, Hidayatullah AR, Hartadi EB, et al. Review on the Pharmacological and Health Aspects of Apium Graveolens or Celery: An Update. Systematic Reviews in Pharmacy, (2021); 12(1): 606-612.
Safira A, Widayani P, An-najaaty D, Rani CAM, Septiani M, et al. A review of an important plants: Annona squamosa leaf. Pharmacognosy Journal, (2022); 14(2): 456-463.
Prihanti GS, Faradilla A, Rahman M. The effect of single type black garlic (Allium sativum L.) extract on the cell of langerhans islets and the kidney tubular microscopy in male wistar rats (Rattus norvegicus) modles of diabetes mellitus. Systematic Reviews in Pharmacy, (2020); 11(6): 290-296.
Al-Malki AL, El Rabey HA. The antidiabetic effect of low doses of Moringa oleifera Lam. seeds on streptozotocin induced diabetes and diabetic nephropathy in male rats. BioMed Research International, (2015); 2015: 1-13.
Paliwal C, Ghosh T, Bhayani K, Maurya R, Mishra S. Antioxidant, anti-nephrolithe activities and in vitro digestibility studies of three different cyanobacterial pigment extracts. Marine Drugs, (2015); 13(8): 5384-5401.
Solikhah TI, Solikhah GP. Effect of Muntingia calabura L. leaf extract on blood glucose levels and body weight of alloxan-induced diabetic mice. Pharmacognosy Journal, (2021); 13(6): 1450-1455.
Haraguchi R, Kohara Y, Matsubayashi K, Kitazawa R, Kitazawa S. New insights into the pathogenesis of diabetic nephropathy: proximal renal tubules are primary target of oxidative stress in diabetic kidney. Acta Histochemica et Cytochemica, (2020); 53(2): 21-31.
Uehara-Watanabe N, Okuno-Ozeki N, Minamida A, Nakamura I, Nakata T, et al. Direct evidence of proximal tubular proliferation in early diabetic nephropathy. Scientific Reports, (2022); 12(1): 778.
Catalani E, Silvestri F, Bongiorni S, Taddei AR, Fanelli G, et al. Retinal damage in a new model of hyperglycemia induced by high-sucrose diets. Pharmacological Research, (2021); 166: 105488.
Ibrahim KA, Abdelgaid HA, El-Desouky MA, Fahmi AA, Abdel-Daim MM. Linseed ameliorates renal apoptosis in rat fetuses induced by single or combined exposure to diesel nanoparticles or fenitrothion by inhibiting transcriptional activation of p21/p53 and caspase-3/9 through pro-oxidant stimulus Environmental Toxicology, (2021); 36(5): 958-974.
Widiastuti EL, Ardiansyah BK, Nurcahyani N, Silvinia A. Antidiabetic potency of jeruju (Acanthus ilicifolius L.) ethanol extract and taurine on histopathological response of mice kidney (Mus musculus L.) induced by alloxan. In Journal of Physics: Conference Series, (2021); 175(1): 012052.
Solikhah TI, Rani CA, Septiani M, Putra YA, Rachmah Q, et al. Antidiabetic of Hylocereus polyrhizus peel ethanolic extract on alloxan induced diabetic mice. Iraqi Journal of Veterinary Sciences, (2022); 36(3): 797-802.
Safitri A, Srihardyastutie A, Roosdiana A, Aulanni'am AA, Octaviana ENL. Effects of root extract of ruellia tuberosa l. on kidneys of diabetic rats. Journal of Mathematical and Fundamental Sciences, (2019); 51(2): 127-137.
Liu CH, Lee TH, Lin YS, Sung PS, Wei YC, Li YR. Pioglitazone and PPAR-γ modulating treatment in hypertensive and type 2 diabetic patients after ischemic stroke: a national cohort study. Cardiovascular Diabetology, (2020); 19(1): 1-13.
Mourougavelou V, Chowdhury TA. Management of hyperglycaemia in people with obesity. Clinical Medicine, (2023); 23(4): 364-371.
Derangula M, Ruhinaz KK, Panati K, Subramani PA, Tatireddigari VRA, et al. Natural product ligands of the peroxisome proliferator-activated receptor gamma as anti-inflammatory mediators. The Natural Products Journal, (2023); 13(6): 25-39.
DOI: http://dx.doi.org/10.62940/als.v12i2.3616
Refbacks
- There are currently no refbacks.