Autophagy mutant cells accumulate proteins involved in cell proliferation, dehydration and rehydration in Physcomitrium patens

Kyosuke Mukae, Maya Shindo

Abstract


Background: Autophagy is responsible for the degradation of cytoplasmic components in lysosomes and vacuoles. Generally, autophagy contributes to cellular survival against stress, since autophagy-deficient (atg) mutants are predominantly more sensitive to environmental stress, including nutrient starvation, than their WT counterparts are. Nevertheless, we found that compared with WT Physcomitrium patens (P. patens) plants, atg mutants can survive desiccation stress in the presence of ABA; however, the underlying molecular mechanism is not understood.

Methods: To comprehend the molecular mechanism, two distinct analyses (proteomic analysis and microarray expression analysis) were performed on P. patens WT and atg mutant protonemal colonies.

Results: An integrated analysis showed that signaling pathways involved in MYBs and MAPKs were activated and that protective proteins such as LEAs and antioxidant-related proteins were not differentially expressed between the WT and atg mutant. This suggests that the atg mutant is highly tolerant to desiccation stress through the activation of cell proliferation during dehydration and rehydration.

Conclusion: This study has provided evidence that autophagy is involved in the relationship between desiccation tolerance and cell proliferation, but the direct effects remain elusive. Further work is needed to elucidate the dynamics of proteins involved in autophagy and the induction of desiccation tolerance.

Keywords:

Autophagy, Desiccation, Physcomitrium, EBP1, MYB, MAPK

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References


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DOI: https://doi.org/10.62940/als.v13i1.3619

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