Cinnamic acid ameliorates diet-induced hyperlipidemia in Wistar rats through antioxidant and gene expression modulatory effects
Abstract
Background: Diet-induced hyperlipidemia is strongly associated with metabolic disorders and cardiovascular diseases. This study explored the ameliorating effects of cinnamic acid (CA) on high-fat diet (HFD)-induced hyperlipidemia in Wistar rats.
Methods: Male Wistar rats were arranged into four groups based on their feeding pattern: control, HFD, control + CA, or HFD + CA. CA was orally administered every day (50 mg/kg body weight). The feeding was continued for 8 weeks, after which the rats were sacrificed, and oxidative stress-associated parameters such as MDA, NO, AOPP, GSH, SOD, and catalase, lipid profiles, and liver enzyme levels were investigated in the serum. Using hepatic tissues, the mRNA levels of several proteins related to the metabolism, uptake, transportation, and storage of lipid was also explored.
Results: Administration of CA significantly (p<0.05) mitigated HFD-induced oxidative stress and increased hepatic enzyme activity. It also prevented the HFD-induced increased mRNA levels of SREBP-2, PPARγ, HMGCR, and Apo-B100. HFD-mediated suppression of LDLR, ABCA1, and Apo-A1 mRNA was significantly (p<0.05) increased by feeding CA. All these positive effects resulted in the reduction of liver weight, adipose tissue weight, and overall body weight, and pro-atherogenic lipid levels, except TG, along with an increase in HDL cholesterol levels.
Conclusion: CA positively influences HFD-induced hyperlipidemia and adiposity in Wistar rats through its antioxidant, hepatoprotective, and gene expression modulatory effects.
Keywords:
Cinnamic acid, Hyperlipidemia, Adipogenesis, Sterol regulatory element-binding protein, Peroxisome proliferator-activated receptorFull Text:
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DOI: https://doi.org/10.62940/als.v13i1.3958
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