Antimicrobial activities, pollen diversity and physicochemical properties of natural honey from Southeastern Anatolia of Turkey

Menderes Cenet, Adnan Bozdogan, Gokhan Sezer, Leyla Acar, Zeynep Ulukanli


Background: Honey, a natural sweetener, is produced from the nectar of many plants. The pollen diversity, physicochemical properties, and antimicrobial activities were analyzed in honey samples from Mardin (Southeastern Anatolia).

Methods: The melissopalynological method was used to identify and enumerate the pollen granules. Analytical methods and agar well diffusion assays were employed for the determination of some quality parameters and the antimicrobial potential of honey samples, respectively.

Results: The pollen composition consisted of 27 taxa belonging to 13 families. The origins of all honey were determined as the multifloral sources. The most predominant taxa were mainly Hedysarum sp., Carduus sp., Melissa officinalis, Gossypium hirsitum, Paliurus spina-christi, Salix sp. and Pimpinella anisum. The secondary pollen taxa were Hedysarum sp., Trifolium sp., Astragalus sp., Salix sp., Paliurus spina-christi, Asphodeline sp., Centaurea sp., Carduus sp., Zea mays and Cistus sp., respectively. Asphodeline sp. as a secondary pollen taxon in a honey sample could be considered as the first report. The pH, total acidity, brix, refractive index, electrical conductivity, moisture and L, a, b values of the samples varied from 3.75 to 4.28, 30 to 42, 67.3 to 85.70, 1.45 to 1.50, 12.40 to 31.61, 0.24 to 0.90, 47.81 to 57.59, -0.94 to 4.31, 20.37 to 31.28, respectively. Antimicrobial activities of the honey specimens were also effective on five bacterial species and two yeast species.

Conclusions: Honey samples from Southeastern Anatolia revealed a good diversity of pollen granules. The rich multiflora of honey increases not only its nutritional quality as well as antimicrobial potential on various clinically important microorganisms. 

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Küçük M, Kolaylı S, Karaoğlu Ş, Ulusoy E, Baltacı C, et al. Biological activities and chemical composition of three honeys of different types from Anatolia. Food Chemistry, (2007); 100(2): 526-534.

Sorkun K, Inceoglu O. Secondary pollens in honey of the central Anatolia region. Doga Bilim Dergisi, (1984); 8382-384.

Mercan N, Guvensen A, Celik A, Katircioglu H. Antimicrobial activity and pollen composition of honey samples collected from different provinces in Turkey. Natural Product Research, (2007); 21(3): 187-195.

Maurizio A, Hodges F. Pollen analysis of honey. Bee World, (1951); 32(1): 1-5.

Louveaux J, Maurizio A, Vorwohl G. Methods of melissopalynology. Bee world, (1978); 59(4): 139-157.

Çenet M, Toroğlu S, Keskin D, Bozok F. Pollen analysis and antimicrobial properties of honey samples sold in Western Turkey. Pakistan Journal of Zoology, (2015); 47(1): 269-273.

Gomes S, Dias LG, Moreira LL, Rodrigues P, Estevinho L. Physicochemical, microbiological and antimicrobial properties of commercial honeys from Portugal. Food and Chemical Toxicology, (2010); 48(2): 544-548.

Ulukanli Z, Oz AT, Cenet M. The authenticity of honey and its effect on strawberry fruits. Journal of Food Processing and Preservation, (2012); 36(4): 364-373.

Cenet M, Ulukanli Z, Bozdogan A, Sezer G, Memis E. The authentication of the botanical origin, physicochemical properties, antioxidant and antimicrobial activities of East Mediterranean honey. Biointerface Research in Applied Chemistry, (2016); 6(6).

Kahraman T, Buyukunal SK, Vural A, Altunatmaz SS. Physico-chemical properties in honey from different regions of Turkey. Food Chemistry, (2010); 123(1): 41-44.

Cano C, Felsner M, Matos J, Bruns R, Whatanabe H, et al. Comparison of methods for determining moisture content of citrus and eucalyptus Brazilian honeys by refractometry. Journal of Food Composition and Analysis, (2001); 14(1): 101-109.

de Rodrı́guez GO, de Ferrer BS, Ferrer A, Rodrı́guez B. Characterization of honey produced in Venezuela. Food Chemistry, (2004); 84(4): 499-502.

Tornuk F, Karaman S, Ozturk I, Toker OS, Tastemur B, et al. Quality characterization of artisanal and retail Turkish blossom honeys: Determination of physicochemical, microbiological, bioactive properties and aroma profile. Industrial Crops and Products, (2013); 46124-131.

Yücel Y, Sultanog P. Characterization of honeys from Hatay Region by their physicochemical properties combined with chemometrics. Food Bioscience, (2013); 116-25.

Anklam E. A review of the analytical methods to determine the geographical and botanical origin of honey. Food Chemistry, (1998); 63(4): 549-562.

Silva LR, Videira R, Monteiro AP, Valentão P, Andrade PB. Honey from Luso region (Portugal): Physicochemical characteristics and mineral contents. Microchemical Journal, (2009); 93(1): 73-77.

Terrab A, Recamales AF, Hernanz D, Heredia FJ. Characterisation of Spanish thyme honeys by their physicochemical characteristics and mineral contents. Food Chemistry, (2004); 88(4): 537-542.

Corbella E, Cozzolino D. Classification of the floral origin of Uruguayan honeys by chemical and physical characteristics combined with chemometrics. LWT-Food Science and Technology, (2006); 39(5): 534-539.

Ahmed J, Prabhu S, Raghavan G, Ngadi M. Physico-chemical, rheological, calorimetric and dielectric behavior of selected Indian honey. Journal of Food Engineering, (2007); 79(4): 1207-1213.

Juszczak L, Socha R, Rożnowski J, Fortuna T, Nalepka K. Physicochemical properties and quality parameters of herbhoneys. Food Chemistry, (2009); 113(2): 538-542.

Terrab A, Dı́ez MJ, Heredia FJ. Characterisation of Moroccan unifloral honeys by their physicochemical characteristics. Food Chemistry, (2002); 79(3): 373-379.

Belay A, Solomon W, Bultossa G, Adgaba N, Melaku S. Physicochemical properties of the Harenna forest honey, Bale, Ethiopia. Food chemistry, (2013); 141(4): 3386-3392.

Beretta G, Granata P, Ferrero M, Orioli M, Facino RM. Standardization of antioxidant properties of honey by a combination of spectrophotometric/fluorimetric assays and chemometrics. Analytica Chimica Acta, (2005); 533(2): 185-191.

Zhao X, Zhang C, Guigas C, Ma Y, Corrales M, et al. Composition, antimicrobial activity, and antiproliferative capacity of anthocyanin extracts of purple corn (Zea mays L.) from China. European Food Research and Technology, (2009); 228(5): 759-765.

Pop C, Vodnar D, RANGA F, Socaciu C. Comparative antibacterial activity of different plant extracts in relation to their bioactive molecules, as determined by LC-MS analysis. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Animal Science and Biotechnologies, (2013); 70(1): 86-94.



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