Correction: Identification of BlaTEM Gene Encode Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli Isolated from Fresh Beef

Abstract

This Correction refers to:

Maghfiroh L, Effendi MH, Tyasningsih W, Witaningrum AM, Bernard AN (2024). Identification of BlaTEM Gene Encode Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli Isolated from Fresh Beef. Advancements in Life Sciences, 11(4):741747. https://doi.org/10.62940/als.v11i4.1800

Following a post-publication editorial quality review, the Editorial Office identified citation inconsistencies involving References [6], [32], [38], [39], and [40]. The authors reviewed the cited sources and submitted revisions to References [6], [32], and [38]. References [39] and [40] were retained following clarification of their intended supporting roles. Related wording in the Discussion was also revised to better align the statements with the cited literature.

Reference and text corrections

• Ref. [6] Reference [6], cited in Table 1 in connection with the blaTEM PCR primer information, was revised. The previous reference concerning prevalence and antibiotic resistance of Staphylococcus aureus and Escherichia coli isolated from raw milk was replaced with a reference concerning genetic identification of blaCTX-M and blaTEM genes in ESBL-producing Escherichia coli.
• Ref. [32] Reference [32], cited in connection with the reported Aztreonam resistance finding in Escherichia coli from raw milk, was revised. The previous reference concerning Shiga toxin-encoding genes in multidrug-resistant Escherichia coli was replaced with a reference concerning molecular identification of blaCTX-M and blaTEM genes in ESBL-producing Escherichia coli isolated from raw cow's milk.
• Ref. [38] Reference [38], cited together with References [39] and [40] in the discussion of antimicrobial resistance in food-producing animals and livestock production systems, was revised. The previous review concerning salmonellosis on poultry farms was replaced with a study addressing multidrug resistance and ESBL-producing Escherichia coli from dairy farms.
• Refs. [39] and [40] These references were reviewed and retained. Reference [39] concerns multidrug-resistant Klebsiella pneumoniae isolated from poultry farms, whereas Reference [40] concerns tetracycline resistance in Streptococcus agalactiae isolated from bovine subclinical mastitis. The surrounding Discussion text was revised to more accurately reflect the supporting scope of References [3840].

Discussion text

The relevant Discussion text was revised from:

The most prevalent beta-lactamase seen in Gram-negative bacteria is the blaTEM gene. 90% of Escherichia coli's resistance to ampicillin is brought on by the blaTEM gene production [37]. The presence of resistant bacteria in food-producing animals and food products of animal origin is caused by the continuous use of antibiotics for therapeutic and non-therapeutic purposes in the production system [3840]. In addition, fresh beef can be contaminated with antibiotic-resistant pathogens at the point of unhygienic slaughter and sale. Although selective pressure is the main cause while, sanitary conditions at slaughter, sale, and processing sites can influence the intensity pattern of distribution along the food chain [33].

to:

The most prevalent beta-lactamase identified in Gram-negative bacteria is the blaTEM gene. Approximately 90% of Escherichia coli resistance to ampicillin is associated with blaTEM gene production [37]. The presence of resistant bacteria in food-producing animals and food products of animal origin has been reported in different livestock production systems [3840]. Antibiotic exposure during therapeutic and non-therapeutic use may contribute to the selection and dissemination of resistant bacteria [3840]. In addition, fresh beef can be contaminated with antibiotic-resistant pathogens at the point of unhygienic slaughter and sale. Although selective pressure is considered a major driver of antimicrobial resistance, sanitary conditions at slaughter, sale, and processing sites can influence the intensity pattern of distribution along the food chain [33].

Corrected references

Reference [6]

Kristianingtyas L, Effendi MH, Tyasningsih W, Kurniawan F. Genetic identification of blaCTX-M gene and blaTEM gene on extended spectrum beta lactamase (ESBL) producing Escherichia coli from dogs. Indian Veterinary Journal, (2020);97(1):1721.

Reference [32]

Ansharieta R, Ramandinianto SC, Effendi MH, Plumeriastuti H. Molecular identification of blaCTX-M and blaTEM genes encoding extended-spectrum beta-lactamase (ESBL) producing Escherichia coli isolated from raw cow's milk in East Java Indonesia. Biodiversitas, (2021);22(4):16001605.

Reference [38]

Widodo A, Lamid M, Effendi MH, Khairullah AR, Kurniawan SC, et al. Antimicrobial resistance characteristics of multidrug resistance and extended spectrum beta-lactamase producing Escherichia coli from several dairy farms in Probolinggo, Indonesia. Biodiversitas, (2022);23(1):215221.

References [39] and [40] were retained unchanged following clarification of their intended supporting roles.

These corrections involve replacement of References [6], [32], and [38], together with revision of the related Discussion wording. They do not alter the experimental dataset, numerical results, tables, figures, statistical analyses, or principal conclusions of the study.

The Editorial Office thanks the authors for their cooperation in reviewing and correcting the citation issues identified during the post-publication quality review.

Correction Published

03 October 2026: The necessary corrections have been carried out in the Version of Record.