Molecular Characterization of EGLN1 Gene in Fast and Slow Moving Animals of Diverse Terrain

Rashid Saif, Naila Naz, Beenish Tariq, Ali Iftekhar


Background: Process of animal migration from their habitat to a new environment is always problematic due to low adaptive tendency, which ultimately affect their production, behavior and overall performance. Current study is focused to explore the sequence diversity of EGLN1 gene in relation to animal acclimatization at higher altitude under deprived oxygen or ability of better utilization of oxygen which is considered to be liable for their agility in diverse terrains. Fast and slow moving animals of plain and hilly terrains are potential species to study this phenomenon.

Methods: Molecular characterization of EGLN1 gene was performed in sheep, goat, buffalo and camels of Pakistan as slow moving candidate species, while tiger, leopard, wolf, ibex, urial and markhor as fast moving candidate species of diverse terrains by extraction their DNA from whole blood, followed by PCR amplification and sequence analysis of EGLN1 gene through BioEdit software. Later on, certain bioinformatics tools like MEGA, protparam and blast2sequence are used for the characterization of the normal and mutant EGLN1 protein.

Results: Current study revealed that goat and camel showed a nucleotide change at c.810 position. While all fast moving animals of higher altitude showed a nucleotide change at position c.406 except one sample of markhor.

Conclusion: Current study will assist to have an idea of sequence diversity of aforementioned candidate gene responsible for adaption of animals in oxygen deprived environment of diverse terrain and may be responsible for their agile behaviour of movement. 

Keywords: EGLN1, Fast moving animals, Slow moving animals, Animal acclimatization, Behavioural traits, Pakistani animals

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Geng RQ, Wang LP. The PCR-SSCP and DNA sequencing methods detecting genetic mutations of EGLN1 gene in different sheep breeds. Indian Journal of Animal Research, (2015); 49(1).

Shah A, Cadinu D, Henke RM, Lianoglou S, Xin X, et al. Deletion of a Subgroup of Ribosome-related Genes Minimizes Hypoxia-induced Changes and Confers Hypoxia Tolerance in Saccharomyces Cerevisiae. Physiology and Genomics, (2011); 43(14): 855-72.

Bigham AW, Lee FS. Human high-altitude adaptation: forward genetics meets the HIF pathway. Genes & development, (2014); 28(20): 2189-2204.

Semenza GL. Involvement of oxygen-sensing pathways in physiologic and pathologic erythropoiesis. Blood, (2009); 114(10): 2015-2019.

Maxwell PH, Wiesener MS, Chang GW, Clifford SC, et al. The tumour suppressor protein VHL targets hypoxia-inducible factors for oxygen-dependent proteolysis. Nature, (1999); 399(6733): 271.

Shah AN, Alam MM, Cao T, Zhang L. The Role of Ribosomes in Mediating Hypoxia Response and Tolerance in Eukaryotes. MOLECULAR MECHANISMS UNDERLYING HYPOXIA-TOLERANCE AND RESPONSE IN YEAST, (2013); 81.

Tang Q, Huang W, Guan J, Jin L, Che T, et al. Transcriptomic analysis provides insight into high-altitude acclimation in domestic goats. Gene, (2015); 567(2): 208-216.

Ai H, Yang B, Li J, Xie X, Chen H, et al. Population history and genomic signatures for high-altitude adaptation in Tibetan pigs. BMC genomics, (2014); 15(1): 834.

Panariti A, Miserocchi G, Rivolta I. mRNA expression profile of selected oxygen sensing genes in the lung during recovery from chronic hypoxia. Biological research, (2013); 46(2): 169-176.

Cho YS, Hu L, Hou H, Lee H, Xu J, et al. The tiger genome and comparative analysis with lion and snow leopard genomes. Nature Communications, (2013); 4(3433): 1-7.

Keller I, Alexander J, Holderegger R, Edwards P. Widespread phenotypic and genetic divergence along altitudinal gradients in animals. Journal of evolutionary biology, (2013); 26(12): 2527-2543.

Lieb ME, Menzies K, Moschella MC, Ni R, Taubman MB. Mammalian EGLN genes have distinct patterns of mRNA expression and regulation. Biochemistry and cell biology, (2002); 80(4): 421-426.

Maxwell P. HIF-1’s relationship to oxygen: simple yet sophisticated. Cell Cycle, (2004); 3(2): 151-154.

Udpa N, Ronen R, Zhou D, Liang J, Stobdan T, et al. Whole genome sequencing of Ethiopian highlanders reveals conserved hypoxia tolerance genes. Genome biology, (2014); 15(2): R36.

Cheviron Z, Brumfield R. Genomic insights into adaptation to high-altitude environments. Heredity, (2012); 108(4): 354.

West JB. High-altitude medicine. American journal of respiratory and critical care medicine, (2012); 186(12): 1229-1237.

Fong G, Takeda K. Role and regulation of prolyl hydroxylase domain proteins. Cell death and differentiation, (2008); 15(4): 635.

Marchler-Bauer A, Zheng C, Chitsaz F, Derbyshire MK, Geer LY, et al. CDD: conserved domains and protein three-dimensional structure. Nucleic acids research, (2012); 41(D1): D348-D352.

Idicula-Thomas S, Balaji PV. Correlation between the structural stability and aggregation propensity of proteins. In silico biology, (2007); 7(2): 225-237.

Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular biology and evolution, (1993); 10(3): 512-526.


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